208 



THE ACTINOMYCETES, Vol. II 



rarely more than 2 to 3 coils. Spores oval to 

 elliptical, sometimes spherical, 1.2 to 2.8 by 

 0.8 to 1.5 /i. 



Sucrose nitrate agar: Growth thin, color- 

 less, covered with sandy lavender to dark 

 gray aerial mycelium. Reverse of growth 

 light gray, later becoming grayish-yellow. 

 No soluble pigment. 



Nutrient agar: Growth cream-colored. 

 Aerial mycelium white. No soluble pigment. 



Calcium malate glycerol: Growth good, 

 raised in center. Aerial mycelium gray, buff 

 around the edges, having a fuzzy appearance. 



Glucose agar: Growth smooth, yellowish, 

 covered with white to gray aerial mycelium. 

 Yellowish soluble pigment. 



Potato: Growth abundant, lichenoid, 

 cream-colored. Aerial mycelium abundant, 

 cottony, white to gray. Color of plug be- 

 comes reddish-tan. 



Gelatin: Cream-colored ring, covered with 

 white aerial mycelium. Rapid liquefaction. 

 Faint yellow soluble pigment. 



Milk: Cream-colored to brownish ring. 

 Coagulation and rapid peptonization. 



Starch: Growth brownish. Aerial myce- 

 lium mouse-gray. Diastatic action strong. 



Production of H 2 S: Negative. 



Antagonistic properties: On certain com- 

 plex nitrogenous media, the organism shows 

 a wide range of antimicrobial activity, 

 partly because of borrelidin. 



Remarks: Morphologically, the culture 

 resembles S. albidoflavus, S. calif ornicus, S. 

 lipmanii, and certain others, but it is not 

 believed to be identical to any of them. 

 Ettlinger et al. (1958) considered this or- 

 ganism as a strain of S. fradiae. Okami and 

 Suzuki (1958) could not demonstrate any 

 spirals on several media tested. 



Type culture: IMRU 3602; ATCC 10,739. 



210. Streptomyces roseochromogenes (Jen- 

 sen, 1931) Waksman and Henrici, 1948 

 (Jensen, H. L. Proc. Linnean Soc. X. S. 

 Wales 56: 359, 1931). 



Waksman and Curtis (1916) and Waks- 



man (1919) described an organism as .1. 

 roseus Krainsky. This culture was, in con- 

 trast to Krainsky's organism, chromogenic. 

 Jensen (1931) compared it with his own 

 isolates and changed the name .4. roseus to 

 roseochromogenes, because of the fact that 

 the name roseus had previously been used by 

 Namyslowsky (1912). 



Morphology: Sporophores form numerous 

 open and closed sinistrorse spirals; some- 

 times 3 to 5 branches issue together from 

 end point of main stem, giving impression 

 of brooms or verticils. Spores spherical, 1.0 

 to 1.2 by 1.3 to 3.0 M (PL V, 2b). 



Sucrose nitrate agar: Growth thin, spread- 

 ing, colorless to pale yellow. Aerial mycelium 

 pale grayish-rose. 



Glucose-asparagine agar: Growth pale 

 yellow. Aerial mycelium white, later be- 

 coming rose-cinnamon, with many small 

 white tufts. 



Nutrient agar: Growth wrinkled, yellow- 

 ish-gray, later brown-red. Aerial mycelium 

 white, then rose-gray. Soluble pigment deep 

 brown. 



Potato: Growth wrinkled, yellowish-gray 

 to grayish-black. Aerial mycelium absent or 

 white. Soluble pigment black. Melanin- 

 positive. 



Gelatin: Colonies small, cream-colored, in 

 bottom of liquefied zone. Soluble pigment 

 blown. Liquefaction medium. 



Milk: Coagulation limited; peptonization 



slow. 



Starch media: Growth colorless, spreading. 

 Hydrolysis good. 



Nitrate reduction: Positive. 



Production of IPS: Positive. 



Antagonistic properties: Active against 

 various bacteria; produces antibiotic roseo- 

 mycin. 



Habitat: Soil. 



Remarks: Jensen (1931) obtained, on 

 plating the tufts of white aerial mycelium 

 arising on agar media, a variant with pure 

 white aerial mycelium. 



Type culture: ATCC 13,400. 



