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THE ACTINOMYCETES, Vol. II 



Figure 44. Verticil formation by S. roseover- 

 ticillatus (Reproduced from: Shinobu, R. Mem. 

 < >saka Univ. Ser. B, No. .">, p. 93, 1956). 



Hum limited to center of colonies, white to 

 rose. 



Starch agar: Growth golden yellow. Aerial 

 mycelium whitish. 



Potato: Growth yellow. No aerial myce- 

 lium. No soluble pigment. 



Gelatin: Liquefaction strong. Colonies at 

 bottom of liquefied zone orange-brown. No 

 soluble pigment. Melanin-negative. 



Milk: Ring cream-colored. Coagulation 

 and peptonization rapid, medium becoming 

 strongly alkaline. 



Cellulose: Growth on paper fair; cellulose 

 decomposed. 



Nitrate reduction: Positive. 



Production of H 2 S: Negative. 



Antagonistic properties: Produces a basic 

 antibiotic, flavomycin, similar to neomycin. 



Remarks: Culture similar to S. micro- 

 flavus. Gause et al. (1957) described other 

 closely related cultures, such as A. roseoful- 

 vis. 



Type culture: [MRU 3672 ; ATCC 13,1(17. 



214. Streptomyces roseoverticillatus Shin- 

 obu, 1956 (Shinobu, R. Mem. Osaka Univ., 

 B (N.S.)5:84 93, L956). 



Morphology: Sporophores produce abun- 



dant primary and secondary verticils (Fig. 

 44) . Spores spherical to elliptical, 0.8 to 1.0 /*. 



Sucrose nitrate agar: Growth thin, mod- 

 erate, pinkish-red. Aerial mycelium cottony, 

 pink to pale pink. 



Glucose-asparagine agar: Growth red to 

 purple-red. Aerial mycelium cottony, dull 

 red to reddish-brown. 



Nutrient agar: Growth reddish-brown to 

 deep brown. Aerial mycelium thin, pinkish 

 to red. Soluble pigment reddish-brown to 

 deep brown. 



Potato: Growth brownish-red to dull red. 

 Aerial mycelium powdery, pink to reddish- 

 purple. Soluble pigment brown. 



Milk: Growth red. Aerial mycelium pale 

 pink. Coagulation and peptonization strong. 

 Soluble pigment pale brown. 



Gelatin: Rapid liquefaction. 



Starch: Rapid hydrolysis. 



Tyrosinase reaction: Weak. 



Cellulose: No growth. 



Nitrate reduction: Positive. 



Carbon utilization: Utilizes fructose; 

 inositol uncertain. Does not utilize xylose, 

 rhamnose, sucrose, lactose, raffinose, or 

 mannitol. 



Habitat: Soil in Japan. 



Remarks: Resembles S. rubrireticuli. 



215. Streptomyces roseus (Namyslowsky, 

 1909; emend. Krainsky, 1914; emend. Waks- 

 man and Curtis, 1916) Waksman and 

 Henrici, 1948 (Namyslowsky, B. Centr. 

 Bakteriol. Parasitenk. Abt. I, Orig. 62: 564, 

 1909; Krainsky, A. Centr. Bakteriol. Para- 

 sitenk. Abt. II, 41: 082-683, 1914; Waks- 

 man, S. A. and Curtis, R. E. Soil Sci. 1: 125, 

 1910). 



Morphology: Sporophores produce num- 

 erous open and closed dextrorse spirals. Ac- 

 cording to Okami, sporophores are straight, 

 without spirals. Spores oval to elongate, 1.5 

 to 2.0 by 1.1 fi. 



Sucrose nitrate agar: Growth colorless. 

 Aerial mycelium pale brownish-vinaceous. 

 No soluble pigment. 



