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THK ACTINOMYCETES, Vol. II 



to brown. No soluble pigment. Melanin- 

 negative. 



Gelatin: Surface growth coral-pink. No 

 liquefaction. No soluble pigment. 



Milk: Growth coral-pink. Aerial mycelium 

 powdery, white. No coagulation and no 

 peptonization. Soluble pigment sometimes 

 slightly reddish. 



Starch: No hydrolysis. 



Nitrate reduction: None. 



Production of H 2 S: Negative. 



Carbon utilization: Glycerol and glucose 

 utilized, but not other carbohydrates. 



Antagonistic properties: Produces an anti- 

 viral agent, abikoviromycin. 



Remarks: .4. griseoruber of Gause et al. 

 (1957) appears to be a closely related form. 

 This organism is considered by R. Gordon as 

 a Nocardia, related to N. asteroides. 



Type culture: IMRU 3655. 



218. Streptomyces rubrireticuli (Waksman, 

 L919) Waksman and Henrici, 1948 (Waks- 

 man, S. A. Soil Sci. 8: 146, L919). 



Synonyms: Actinomyces, reticulus-ruber 

 Waksman. L919; A. reticulus Bergey, 2nd 

 ed., 1925. 



Morphology: Sporophores produce both 

 primary and secondary verticils; composi- 

 tion of medium influences structure of 

 sporophores, glucose-asparagine agar favor- 

 ing spiral formation. Spores oval-shaped, 

 smooth (PI. I a). 



Sucrose nitrate agar: Growth abundant, 

 spreading, usually pink. Aerial mycelium 

 white, later rose to pink. 



Glucose-asparagine agar: Entire growth 

 abundant, spreading, rose-red. 



Nutrient agar: Growth red, with yellowish 

 margin, becoming red. Soluble pigment dark 

 brown. 



Starch agar: Growth white with red tinge. 

 Hydrolysis fair. 



Potato: Growth cream-colored, later pink 

 to dark red. Melanin-positive. 



Gelatin: Surface growth yellowish-red to 

 pink. Ready liquefaction. Brown pigment. 



Milk: Growth abundant. Coagulation and 

 peptonization. 



Invertase: Positive. 



Cellulose: Growth good. 



Nitrate reduction: Rapid. 



Production of H 2 S: Positive. 



Antagonistic properties: Certain strains 

 produce an antibiotic designated as streptin; 

 others produce trichonin. 



Habitat: Soil. 



Remarks: Numerous cultures that pro- 

 duce a rose to pink substrate growth, a 

 soluble brown pigment in organic media, and 

 both primary and secondary verticils in the 

 sporophores have been described. It is suf- 

 ficient to mention A. biverticillatus by Gause 

 et al. (1957). 



Type culture: IMRU 3631. 



219. Streptomyces rutgersensis (Waksman 

 and Curtis, 1916) Waksman and Henrici, 

 1948 (Waksman, S. A. and Curtis, R. E. 

 Soil Sci. 1: 123, 1916; 8: 152, 1919). 



Morphology: Sporophores produce abun- 

 dant close and open spirals. Spores spherical 

 and oval, 1.0 to 1.2 /x. with tendency to bi- 

 polar staining. 



Sucrose nitrate agar: Growth thin, color- 

 less, becoming brownish to almost black. 

 Aerial mycelium white, becoming dull gray. 



Glucose-asparagine agar: Growth abun- 

 dant, brown, becoming black with cream- 

 colored margin. No aerial mycelium appears 

 within 15 days. 



Nutrient agar: Growth thin, wrinkled, 

 cream-colored. 



Starch agar: Growth gray, spreading. 

 Hydrolysis good. 



Potato: Growth abundant, much folded. 

 Aerial mycelium white-gray. Melanin-nega- 

 tive. 



Gelatin: Growth cream-colored. Liquefac- 

 tion medium. No soluble pigment. 



Milk: Cream-colored ring. Coagulation 

 and slow peptonization. 



Cellulose: Growth scant. 



Sucrose: Inversion weak. 



