)ESCRIPTION OF SPECIES OF STREPTOMYCES 



27:; 



Nitrate reduction : ( rood. 



Production of IPS: Negative. 



Temperature: Optimum 37°C. 



Antagonistic properties: Various strains 

 produce xanthomycin-like substances; others 

 produce ruticin. 



Remarks: The pigmenl formed is not 

 soluble. Krassilnikov (1949) considered the 

 organism, quite incorrectly, as a variety of 

 .1 . chromogenes. 



Type culture: [MRU 3350. 



220. Streptomyces sahachiroi Hat a et r//., 

 1954 (Ilata, T., Koga, F., Sano, Y., Kana- 

 mori, K., Matsumae, A., Sugawara, R., 

 Hoshi, T., and Shima, T. J. Antibiotics 

 (Japan) 7A: 107-112, 1954). 



.Morphology: Sporophores form numerous 

 closed spirals with a iv\\ open spirals. Spores 

 oval or cylindrical, 0.7 to 1.3 by 0.5 to 0.8 l x. 



Sucrose nitrate agar: Growth folded, dark 

 orange. Aerial mycelium velvety, white to 

 pale grayish-brown. Soluble pigment yel- 

 lowish-brown. 



Calcium malate agar: ( irowth thin, cream- 

 colored with orange-yellow reverse. Aerial 

 mycelium thin, white, powdery. Soluble pig- 

 ment pale orange-yellow. 



Nutrient agar: Growth glistening, white- 

 gray. Xo aerial mycelium. Soluble pigment 

 light brown to yellow. 



Starch agar: ( Irowth thin, yellowish-white. 

 Aerial mycelium thin, powdery, pale red- 

 brown. Xo soluble pigment. Slow hydrolysis. 



Potato: Growth wrinkled, pale yellowish- 

 brown. Aerial mycelium thin, white. Soluble 

 pigment absent or faint brown. 



Gelatin: Growth limited, white. Xo aerial 

 mycelium. Xo soluble pigment. 



Milk: Surface growth white to pale yellow. 

 Coagulation; no peptonization. Strongly 

 alkaline. Soluble pink pigment. 



Nitrate reduction: Positive. 



Carbon utilization: Xylose, arabinose, 

 lactose, trehalose, mannitol, sucrose, salicin, 

 glucose, maltose, mannose, glycerol, dextrin, 

 fructose, starch, galactose, sorbitol utilized. 



Rhamnose, raffinose, inositol, esculin, dulci- 



tol, inulin, sodium acetate, sodium citrate, 

 sodium succinate not utilized. 



Antagonistic properties: Produces anti- 

 tumor agent carzinophilin. 



221. Streptomyces sampsonii (Millard and 

 Burr, 1926) Waksman (Millard, \Y. A. and 

 Burr, S. Ann. Appl. Biol. 13: 580, 1926). 



Morphology: Aerial mycelium produces 

 long, straight sporophores, rarely spiral- 

 shaped. Spores cylindrical, 0.8 to 1.0 by 0.5 

 fx (spores oval to spherical, Waksman and 

 Gordon). 



Sucrose nitrate agar: Growth wrinkled, 

 pale gray to white. Aerial mycelium very 

 scant, white. Soluble pigment green to buff. 



Potato: Growth wrinkled, grayish. Aerial 

 mycelium white. Soluble pigment golden 

 brown (none, Waksman and Gordon). 



Gelatin: Surface growth scant, gray. 

 Aerial mycelium trace, whitish. Liquefaction 

 rapid. Melanin-negative. 



Milk: Surface growth good, whitish. Xo 

 aerial mycelium. No coagulation; no pep- 

 tonization (rapid peptonization, Waksman 

 and Gordon). 



Starch: Xo hydrolysis (rapid hydrolysis, 

 Waksman and Gordon). 



X'itrate reduction: Positive. 



Tyrosinase reaction: Negative. 



Temperature: 28°C. 



Habitat: Potato scab. 



Type culture: [MRU 3371. 



222. Streptomyces sayamaensis Arishima 

 et al, 1955 (Arishima, M., Sekizawa, A., Sato, 

 T.,and Miwa, K. J. Agr. Chem. Soc. Japan 

 29:810-817, 1955). 



Morphology: Sporophores straight, spores 

 short rods to cylindrical, 1.0 by 1.5 m- 



Sucrose nitrate agar: Growth pale yellow. 

 Aerial mycelium gray with brownish tinge. 

 Soluble pigment yellow. 



Glucose-asparagine agar: Growth white to 

 pale yellow, turning pale brown. Aerial my- 

 celium white, becoming pale brown. 



Calcium malate agar: (Irowth limited. 



