280 



THE ACTINOMYCKTES, Vol. II 



(Okami, Y. Taxonomic study of antibiotic 

 streptomyces. Thesis, Hokkaido University, 

 Japan, 1952). 



Morphology: Aerial hyphae with few 

 branches. No spirals, bu1 verticils produced 

 occasionally, depending on composition of 

 medium. 



Glycerol nitrate agar: Growth yellowish- 

 brown, penetrates deep into medium. Aerial 

 mycelium thin, white, with dark yellowish 

 tinge. Soluble pigment yellowish -brown. 



Nutrient agar: Growth wrinkled, yellow- 

 ish-brown. Aerial mycelium scant, white. 

 Soluble pigment slight, yellowish-brown. 



Starch agar: Growth thin, cream-colored. 

 No aerial mycelium. No hydrolysis. 



Gelatin: Growth yellowish-brown at bot- 

 tom of liquefied portion. No aerial mycelium. 

 Soluble pigment slight, yellowish-brown. 

 Slow liquefaction. 



Potato: Growth wrinkled, cream to yel- 

 lowish. No aerial mycelium. Soluble pigment 

 slight, yellowish-brown. 



Milk: Growth on surface of milk yellowish. 

 Aerial mycelium scant. Coagulation occurs 

 in 2 to 3 days, followed by slow peptoniza- 

 tion. 



Blood agar: Growth dark gray with green- 

 ish tinge. Aerial mycelium dark. No hemoly- 

 sis. 



Nitrate reduction: Negative. 



Production of H 2 S: Negative. 



Antagonistic properties: Produces anti- 

 fungal substance, aureothricin (Maeda, 

 1953). 



Type culture: ATCC 12,310. 



235. Streptomyces tumuli (Millard and 

 Beeley, 1927) Waksman (Millard, W. A. 

 and Beeley, F. Ann. Appl. Biol. 14: 296-311, 

 1927). 



Sucrose nitrate agar: Growth gray, later 

 becoming opaque dark. Aerial mycelium 

 arises at center of growth, at first white, 

 later becoming pale gray. Surface of growth 

 covered with colorless drops leaving small 

 black craters. No soluble pigment. 



Glucose-asparagine agar: Growth wrin- 

 kled, pale gray. Aerial mycelium white, 

 arising in concentric rings around a dark 

 bare center. Soluble pigment olive-colored. 



Nutrient agar: Growth good, lustrous, 

 slimy, gray. No aerial mycelium. No soluble 

 pigment. Melanin-negative. 



Potato: Growth heavy, slimy, black. No 

 aerial mycelium. Soluble pigment grayish- 

 brown. 



Gelatin: Growth beaded. No aerial myce- 

 lium. Liquefaction rapid. No soluble pig- 

 ment. 



Milk: Growth good; no aerial mycelium. 

 Coagulation and slight peptonization. 



Starch: Hydrolysis. 



Nitrate reduction: Positive. 



Habitat: Mound scab of mangels. 



236. Streptomyces venezuelae Ehrlich et al., 

 1948 (Ehrlich, J., Gottlieb, D., Burkholder, 

 P. R., Anderson, L. E., and Pridham, T. G. 

 J. Bacterid. 56: 407 -477, 1948; Pridham, 

 T. G. and Gottlieb, D. J. Bacteriol. 56: 

 107-114, 1948). 



Morphology : Sporophores monopodially 

 branched, straight or slightly and irregularly 

 curved. Spores oval to oblong, 0.4 to 0.9 by 

 0.7 to 1.6 /i, smooth (PI. II p). 



Sucrose nitrate agar: Aerial mycelium 

 light lavender. 



Nutrient agar: Substrate growth yellow 

 to brown. Aerial mycelium gray. Soluble 

 pigment dark brown to black. 



Calcium malate agar: Substrate growth 

 yellow to brown; aerial mycelium gray. 



Glucose agar: Soluble pigment dark 

 brown. 



Potato: Growth abundant, gray to dark 

 brown. Aerial mycelium gray. Soluble pig- 

 ment dark brown or black. 



Gelatin: Liquefaction rapid. Soluble pig- 

 ment dark brown. 



Milk: Peptonization. Soluble pigment 

 dark brown. 



Starch agar: Growth white to lavender. 

 Hydrolysis. 



