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THE ACTINOMYCETES, Vol. II 



Starch agar: Growth thin, colorless. Aerial 

 mycelium rose to lavender-colored. Hy- 

 drolysis. 



Potato: Growth abundant, spreading, 

 brownish. Aerial mycelium grayish-pink to 

 lavender. Browning of the potato. 



Gelatin: Surface pellicle gray to brownish. 

 Aerial mycelium thin, white. Soluble pig- 

 ment brown. Liquefaction slow. 



Milk: Growth brown. Coagulation none; 

 peptonization slow. Milk becomes dark gray- 

 brown or black. 



Nitrate reduction: Limited or absent. 



Production of H 2 S: Positive. 



Carbon utilization: Utilizes glucose, man- 

 nose, galactose, maltose, starch, glycerol, 

 sodium acetate, sodium citrate. Does not 

 utilize xylose, lactose, sucrose, mannitol, 

 sorbitol, potassium sodium tartrate. 



Antagonistic properties: Produces an 

 antibiotic, actithiazic acid. 



Remarks: Various related organisms have 

 been listed. It is sufficient to mention A. 

 gobitricini, A. roseolus, A. syringini, and A. 

 roseolilacinus, described by Cause el al. 

 (1957). 



Type culture: IMRU 3051. 



244. Streptomyces viridans (Krassilnikov, 

 1 ( .)41) (Krassilnikov, X. A. Actinomycetales. 

 [zvest. Akad. Xauk. SSSR, Moskau, 1941). 



Morphology: Sporophores branched, spi- 

 ral-shaped. Spores cylindrical. 



Glycerol nitrate agar: Growth olive-green 

 with soluble green pigment. Aerial mycelium 

 dark gray, olive-colored, or gray-green, vel- 

 vety, covering the whole growth. 



Nutrient agar: Growth brown-green. Sol- 

 uble pigment brownish. Melanin-negative. 



Potato: Growth brown. Aerial mycelium 

 light gray. Soluble pigment olive-green 

 (Hoffmann, 1958). 



Gelatin: Rapid Liquefaction. 



Milk: Xo coagulation; rapid peptoniza- 

 tion; soluble brown pigment. 



Starch: Hydrolysis rapid. 



Cellulose: Growth poor. 



Nitrate: Reduction to nitrite. 



Sucrose: Inversion rapid. 



Antagonistic properties: None; some 

 si rains are weakly active. 



Remarks: Related to S. intermedins. 

 Drechsler (1919) described two similar 

 strains, Xos. X and XIV. Gause et al. 

 ( 1957) described a related strain as A. roseo- 

 viridis. 



245. Streptomyces viridis (Lombardo-Pel- 

 legrino, 1903) Waksman (Lombardo-Pelle- 

 grino, P. Riforma med. 39: 1005-1067, 1903. 

 Summarized by Baldacci, E. Atti ist. botan. 

 "Giovanni Briosi" e lab. crittogam. nniv. 

 Pavia (Ser. IV) 11: 221-223, 1939). 



Morphology: Sporophores long or short, 

 straight, undulated; frequently producing 

 broom-shaped clumps. Spores ovoid, 0.7 to 

 1 .4 n in diameter. 



Agar media: Substrate growth on all media 

 at first hyaline, later turning green to dark 

 green. Soluble pigment green. The cultures 

 also grow under anaerobic conditions, but 

 produce no soluble pigment. Aerial mycelium 

 on all media cottony, whitish to grayish. 



Potato: Growth dark violet. Aerial my- 

 celium white. Melanin-negative. 



Production of H 2 S: Positive. 



Antagonistic properties: Not reported, or 

 negative. 



Habitat: Soil. 



Remarks: Baldacci and Comaschi (1956) 

 concluded that the culture described by 

 Krainsky (1914) as .1. griseus belongs more 

 accurately to the S. viridis series. According 

 to Hoffmann (1958), the A. griseus Krainsky 

 appears to belong to this group, although he 

 refers to it as .1. griseus Krassilnikov. It is 

 said to produce broom-shaped sporophores 

 with spirals. Growth colorless, turning light 

 brown. Aerial mycelium velvety, light gray 

 turning dark dray. Xo soluble pigment. 

 Melanin-negative. Growth on potato lichen- 

 oid. Milk not coagulated, but peptonized. 

 Gelatin liquefied. Starch hydrolyzed. It 

 grows on cellulose. 



