290 



THE ACTINOMYCETES, Vol. II 



surface of growth with a mat, yellowish to 

 gray. Soluble pigment brownish or absent. 



Glucose-asparagine agar: Growth moist, 

 yellow to yellow-green. Aerial mycelium 

 abundant, grayish-yellow to sulfur-yellow, 

 later overgrown by white sporulating hy- 

 phae. Soluble pigment absent or faint yellow. 



Nutrient agar: Growth moist, gray to 

 light green with green to almost bluish tinge 

 at bottom of slant. Nonsporulating aerial 

 mycelium appears much later; it is white to 

 gray. No soluble pigment. 



Potato: Growth lichenoid, brownish to 

 greenish-yellow to dark olive-green. Aerial 

 mycelium absent, or formed as thin, yelkrw- 

 ish layer on drier portions of growth. Soluble 

 pigment absent or dark brown. 



Gelatin: Growth in form of surface ring, 

 canary-yellow. Slight liquefaction. Soluble 

 pigment brown to dark brown, a property 

 that may be lost on cultivation. 



Starch : Hydrolysis. 



Cellulose: Limited growth, no destruction 

 of cellulose. 



Production of H 2 S: Negative. 



Carbon utilization: No growth with su- 

 crose, lactose, or rhamnose; good growth on 

 mannose and glucose. 



Antagonistic properties: Produces an anti- 

 fungal substance, candidin. 



Habitat: Soil. 



Type culture: IMRU 3685. 



248. Streptomyces viridogenes (Millard and 

 Burr, 1926) Waksman (S. viridis of Millard, 

 W. A. and Burr, S. Ann. Appl. Biol. 13: 

 580, 1926). 



Morphology: Sporophores long, sympodi- 

 ally branched, straight. Spores spherical, 

 0.9 M , smooth (PI. II o). 



Sucrose nitrate agar: Growth abundant. 

 Aerial mycelium olive-gray. Soluble pigment 

 greenish-yellow to blackish-green. 



Glucose-asparagine agar: Growth smooth, 

 raised, olive-buff. Aerial mycelium abund- 

 ant, light gray to deep mouse-gray. Soluble 

 pigment yellowish to greenish-yellow. 



Nutrient agar: Growth lichenoid, cream- 

 colored. No aerial mycelium. No soluble 

 pigment. 



Nutrient agar with glucose: Growth gray 

 to black. Aerial mycelium gray. Soluble pig- 

 ment dark brown. 



Starch agar: Growth gray to brown. Aerial 

 mycelium thin, white. Hydrolysis positive. 



Potato: Growth gray to olive-gray. Aerial 

 mycelium either absent or white, turning 

 gray. Soluble pigment brown. 



Gelatin: Growth grayish. Aerial mycelium 

 scant, white to gray. Liquet' action rapid. 

 Soluble pigment light golden brown. 



Milk: Surface growth good. Aerial my- 

 celium scant, white. Coagulation rapid and 

 peptonization gradual. 



Nitrate reduction: Positive. 



Tyrosinase reaction: Negative. 



Temperature: Grows well at 37.5°C. 



Habitat: Potato scab. 



Remarks: Ettlinger et al. (1958) reported 

 that this species is melanin-negative. 



249. Streptomyces wedmorensis (Millard 

 and Burr, 1926) Waksman (Millard, W. A. 

 and Burr, S. Ann. Appl. Biol. 13: 580, 1926). 



Morphology: Sporophores straight, 

 branched. Spores oblong, 0.8 to 0.9 by 0.6 to 

 0.8/x- 



Sucrose nitrate agar: Growth flat, thin, 

 grayish. Aerial mycelium white to gray. 



Nutrient potato agar: Growth wrinkled, 

 grayish. No aerial mycelium. Melanin- 

 negative. 



Potato: Growth wrinkled, grayish. Aerial 

 mycelium white. Plug pigmented drab. 



Gelatin: Growth fair. No aerial mycelium. 

 Liquefaction medium. 



Milk: Growth greenish. Coagulation and 

 slow peptonization. 



Starch: Hydrolysis. 



Nitrate reduction: Positive. 



Tyrosinase reaction: Negative. 



Temperature: Grows well at 37.5°C. 



Habitat : Potato tubers. 



