DESCRIPTION OF SPECIES OF STREPTOMYCES 



291 



•_'.')(). Streptomyces willmorei (Erikson 

 L935) Waksman and Eenrici, L948. (Erik- 

 son, I). Med. Research Council (Brit.) 

 Spec. Rept. Ser. No. 203: 19 20, L935). 



Morphology: Submerged growth con- 

 sists of unicellular mycelium frequently 

 branched at short intervals, presenting pe- 

 culiar clubbed and budding forms with 

 occasional separate, round, swollen cells. 

 The filaments are characteristically long 

 homogeneous, and much interwoven. Aerial 

 mycelium profuse in most media, with a 

 marked tendency to produce 1 loose spirals 

 with chains of ellipsoidal spores. Thick aerial 

 clusters may also he formed. 



Glucose-asparagine agar: Growth color- 

 less, wrinkled, confluent, with smooth entire 

 margin; large discrete colonies like flat 

 rosettes. Aerial mycelium scant, white. 



Glycerol nitrate agar: Round, smooth, 

 cream-colored colonies, heavy texture, mar- 

 gin submerged. Stiff, sparse aerial spikes. 



Peptone-beef extract or nutrient agar: 

 Growth heavy, colorless, lichenoid, rounded 

 elevation covered with white aerial myce- 

 lium. Later, submerged margin, round con- 

 fluent growth; aerial mycelium marked in 

 concentric zones. 



Potato agar: Fair growth, partly sub- 

 merged. Aerial mycelium grayish-white. 



Gelatin: Colonies minute, colorless. Posi- 

 tive liquefaction. 



.Milk: Coagulation and slow peptonization. 



Dorset's egg medium: Large, round, color- 

 less, scale-like colonies, radially wrinkled, 

 later growth brownish; medium discolored. 



Serum agar: Smooth colorless discoid col- 

 onies; marked umbilication after 2 weeks. 



Production of H 2 S: Negative. 



Antagonistic properties: Positive. 



Source: Streptothricosis of liver. 



Remarks: Ettlinger et al. (1958) place 

 this species in the S. griseus series. 



Type culture: [MRU 3332. 



251. Streptomyces xanthophaeus Linden- 



,bein, 1952 (Lindenbein, W. Arch. Mikro- 

 l.iol. 17: 361 383, L952). 



Morphology: No description. 



Glycerol nitrate agar: Growth brownish. 

 Aerial mycelium white-gray or reddish-gray. 

 Soluble pigment yellow-brown. 



Glycerol malate agar: Growth deep 

 orange. Aerial mycelium white-gray to red- 

 gray. Soluble pigment dee]) orange. 



Glucose-asparagine agar: Growth diffuse, 

 light yellow. Aerial mycelium white. Solu- 

 ble pigment light yellow. 



Nutrient agar: Growth light brown. Aerial 

 mycelium ash-gray to white. Soluble pig- 

 ment yellow to yellow-brown. Melanin- 

 negative. 



Glucose-peptone agar: Growth light yel- 

 low. Aerial mycelium ash-gray. Soluble pig- 

 ment yellow. 



Starch agar: Growth lichenoid. Aerial 

 mycelium violet -gray. Hydrolysis rapid. 



Potato: Growth lichenoid. Aerial myce- 

 lium gray. No soluble pigment. 



Gelatin: Growth In-own. Aerial mycelium 

 ash-gray. Soluble pigment yellow-brown. 

 Liquefaction strong. 



Milk: Growth lichenoid. Aerial mycelium 

 gray to violet. Soluble pigment dark brown. 

 Peptonization strong. 



Cellulose: No growth. 



Antagonistic properties: Produces geo- 

 mycin, active against gram-negative bac- 

 teria. 



Habitat: Limestone deposit in Germany. 



Remarks: Related to S. erythraeus and 

 S. erythrochromogenes. Kutzner (1956) stud- 

 ied five soil isolates. Four strains did not 

 form any spirals; one did. The spores were 

 smooth. The soluble pigment on glucose- 

 peptone agar was dark brown. He thus con- 

 sidered this species as melanin-positive. 



Addt ikI urn 



After the text of this volume was com- 

 pleted, the following newly described forms 

 appeared in print: 



Streptomyces aerocolonigenes Shinobu and 



