APPENDICES 



329 



K2HPO4 1 .0 gm 



Agar 1 5.0 gm 



Distilled water 1000. ml 



This medium is often known as Conn's. 



6. Glycerol-glydm agar: 



Glycerol 20.0 gm 



Glycine 2.0 gm 



K 2 HP0 4 1.0 gm 



NaCl 2.0 gm 



MgSOwHoO 0.5 gm 



FeS0 4 0.1 gm 



CaC0 3 0.2 gm 



Agar 18.0 gm 



Distilled water 1000.0 ml 



Adjust to pi I 7.2 with NaOH 

 This medium is often known as Plotho's. 



7. Glycerol-calcium malate agar: 



Glycerol 10.0 gm 



Calcium malate 10.0 gm 



NH4CI 0.5 gm 



K..HPO4 0.5 gm 



Agar 15.0 gm 



Distilled water 1000.0 ml 



Glucose or mannitol may be used (20 gm) 

 to replace the glycerol. Calcium citrate may 

 be used to replace the malate. 



8. Glucose-ammonium salt agar: 



Glucose 10.0 gm 



(NH 4 ) 2 HP0 4 4.0 gm 



NaCl 5.0 gm 



K,HP() 4 2.0 gm 



MgS0 4 -7H 2 1.0 gm 



CaCl, 0.4 gm 



FeS0 4 -7H 2 0.02 gm 



MnS() r 7H,() 0.01 gm 



Agar 15.0 gm 



Distilled water 1000.0 ml 



9. Glycerol-ammonium salt agar: 



< Glycerol 10.0 gm 



NH 4 C1 1.0 gm 



K 2 HP0 4 1.0 gm 



MgS0 4 -7H 2 0.5gm 



NaCl 0.5 gm 



CaC0 3 1.0 gm 



Agar 15.0 gm 



Distilled water 1000.0 ml 



pll 7.0 

 The ammonium salt may be replaced by 

 KNO3 . This medium was proposed by 

 Masumoto (1952). 

 10. Glycerol-urea agar: 



( rlycerol 15.0 gm 



Urea 2.0 &m 



KTIPO4 0.5 gm 



MgS0 4 0.5 gm 



NaCl 0.5 gm 



FeS0 4 0.1 gm 



Agar 15.0 gm 



Distilled water 1000.0 ml 



//. Glucose-tyrosine agar: 



Glucose 10.0 gm 



Tyrosine 1.0 gm 



(NH 4 ) 2 S0 4 0.5 gm 



IvdIPO, 0.5 gm 



Agar 15.0 gm 



Distilled water 



Reaction made neutral with Na< )H. 

 12. Glucose-peptone agar: 



Glucose, crude 40 gm 



Peptone 10 gm 



Agar, powdered 20 gm 



Distilled water 1000.0 ml 



pH 5.6 

 Dissolve glucose and peptone in 500 ml dis- 

 tilled water. Dissolve agar, by heating a1 

 100 C, in another 500 ml water. Mix the 

 two solutions, cool, add 10 gm egg-albumin 

 dissolved in 50 ml water. Shake, steam for 

 M0 min, allow clot to settle, filter, distribute 

 in lubes, and autoclave at 1 15°C for 10 min. 

 This medium, also known as Sabouraud's 

 agar, is fairly acid. 

 IS. Tyrosine-casein-nitrate agar: 



Tyrosine 1.0 gm 



Sodium casemate 25.0 gm 



Sodium nitrate 10.0 gm 



Agar 15.0 uin 



Tap water 1000.0 ml 



This medium has been recommended (Men- 

 zies and Dade, 1959) for the isolation of 

 Streptomyces scabies from potato scab lesions 



