APPENDICES 



331 



23. Starch-peptone-beef -agar: 



Starch 10.0 gm 



Peptone 5.0 gm 



Meat extract 3.0 gm 



Agar L5.0 gm 



Distilled water 1000.0 ml 



pH7.0 

 34- Glycerol-starch-glutamaU agar: 



Glycerol 10.0 gm 



Starch 10.0 gm 



Sodium glutamate 1.0 gm 



NaN0 3 0.5 gm 



Proline 0.25 gm 



VitamiD B 0.01 gm 



K2HPO4 0.25 gm 



MgS0 4 0.25 gm 



FeS0 4 0.01 gm 



Agar 15.0 gm 



Distilled water 1000.0 ml 



pH 7.0 to 7.2 



Shinobu (1958b) considered this one of the 



best synthetic media for growth of actino- 

 mycetes, especially for production of aerial 

 mycelium. 



25. Egg-albumin agar: 



( rlucose 10.0 gm 



Egg albumin 0. 15 gm 



K2HPO4 0.5 gm 



.MgSO r 7II,() 0.2 gm 



Fe 2 (S0 4 ) 3 Trace 



Agar 15.0 gm 



Distilled water 1000.0 ml 



Egg albumin is first dissolved in water and 



made neutral to phenolphthalein with X 10 

 NaOH. 



26. Potato-glucose agar: 



Peeled potatoes 200.0 gm 



( rlucose 20.0 gm 



CaC() 3 0.2 gm 



MgS() r 7H 2 0.2 gm 



Agar 15. gm 



Tap or distilled water 1000.0 ml 



pH 6.8 to 7.2 



27. Potato-peptone-glycerol agar: 



Potato 100.0 gm 



Peptone 2.0 gm 



( rlycerol 5.0 gm 



MgSO, 0.5 gm 



K2HPO4 0.5 gm 



XaCl 0.5 gm 



TcSO, 0.01 gm 



Agar 15.0 gm 



Tap water 1000.0 ml 



The potatoes are cut into small cubes to 

 which 100 ml of water is added and the 

 whole steamed for three quarters of an hour. 

 The extract is strained through fine muslin 

 without squeezing the pulp. The other nu- 

 trients are dissolved in 500 ml of water which 

 is then added to the potato extract, and the 

 whole steamed for three quarters of an hour. 

 The mixture is then made up to bulk, stand- 

 ardized and filtered, after which the agar is 

 added. 



28. Glucose-yeast extract-beef -peptom <t(j<ir: 



Glucose 10.0 gm 



Yeast extract 10.0 gm 



Beef extract 4.0 gm 



Peptone 1.0 gm 



XaCl 2.5 gm 



Distilled water 1000.0 ml 



This medium is often known as Emerson's. 

 Various modifications of this medium are 

 used. The concentration of the first two 

 constituents may be reduced to 1.0 gm per 

 liter; the XaCl may be left out. 



29. Glucose- yeast extract agar: 



Glucose 10.0 gm 



Yeast extract 10.0 gm 



Agar 15.0 gm 



Tap water 1000.0 ml 



pH 6.8 



This medium may also contain 0.5 gm 



K.I1PO,. 



80. Glucose-casein digest-yeast-beef agar: 



( rluCOSe 10.0 gm 



Yeast ext ract 1.0 gm 



Beef extract 1.0 gm 



X-Z-Amine A 2.0 gm 



Agar 15.0 gm 



Distilled water 1000.0 ml 



pll 7.:5 



