58 



Journal of Applied Microscopy. 



steps of technical detail involved in an 

 elementary study of such subjects as 

 histology, bacteriology, and pathology. 

 Moreover, for graduates in medicine, 

 identical courses should be offered 

 involving the various methods of a 

 laboratory diagnosis, until the steps 

 in manipulation and the tools required 

 become thoroughly familiar. Post- 

 graduate laboratory courses should be 

 made thoroughly practical in the full 

 sense of the word, and thus attractive; 

 and that army of graduates in medicine 

 who are unfamiliar with laboratory 

 methods should avail themselves of such 

 courses instead of haunting the surgical 

 and gynecologic clinics, as is now the 

 fashion. If more rural practitioners 

 aspired to give their patients the benefit 

 of a thorough diagnosis, and trained 

 themselves for this purpose, the public 

 and the profession would alike be 

 benefited. 



There is no excuse for a physician who 

 does not prepare himself to do the work 

 demanded in routine daily practice. 

 * * * 



The next meeting of the American 

 Microscopical Society will be held in 

 Syracuse, N. Y., August 30th and 31st, 

 and September 1, 1898. 



Syracuse is the central city of New 

 York State, with excellent railroad con- 

 nections in every direction. Its hotels 

 are among the best in the State. It is 

 well known as a convenient city for con- 

 ventions. 



The society is invited to meet in Syra- 

 cuse by the Syracuse Academy of 

 Science. The daily sessions of the society 

 will be held in the fme new building of 

 the College of Medidine, Syracuse Uni- 

 versity. The building is centrally sit- 

 uated, and not far from the hotels. It 

 has commodious lecture rooms admir- 

 ably suited for the work of the soci- 

 ety. Its laboratories are ideal rooms for 

 trade displays of apparatus, or for a 

 working session; and in the evening, for 

 an exhibition soiree, which will probably 

 be made an important part of the gen- 

 eral program. An effort will be made 

 to secure a small special dining room 

 at one of the hotels, for the exclusive use 

 of the society, to contribute to the social 

 enjoyment of the members in attendance. 



The time of this meeting has been so 

 fixed as to make it convenient for mem- 

 bers of the American Society for the 

 Advancement of Science, on leaving Bos- 

 ton, to attend the Syracuse meeting on 

 their homeward journey. 



The Secretary of Agriculture has re- 

 cently issued an order requiring that 

 section directors of the "Weather Buerau 

 shall make themselves familiar with 

 structural and physiological botany. 



ABSTRACTS. 



Method of Injecting the Urinary Tubules, 



etc., of Frog's Kidney. 



O. Frankl. 



For the preparation of a good injec- 

 tion mass the author soaks ten or fifteen 

 sheets of perfectly transparent gelatin 

 in distilled water for twenty-four hours, 

 when the water is drained off and the 

 softened gelatin melted over a water 

 bath, and an equal volume of glycerine 

 is added and warmed with the gelatin; 

 four to five cubic centimeters of a con- 

 centrated solution of corrosive sublimate 

 are stirred in and the whole filtered 

 through fine linen. For coloring the 

 mass a solution of Berlin blue, 1-20, is 

 added and then the mass again filtered 

 through linen. It is injected warm, but 

 the animal or organ need not be warmed. 

 The mass keeps well if a thymol crystal 

 be added. 



The frog should be killed with chloro- 

 form, the abdomen opened widely, and 

 then the frog laid in normal salt solu- 

 tion. For the injection the mass is 

 warmed and the cannula inserted in the 

 canal of Leydig (Ureter; Ductus uro- 

 spermaticus) and the injection made 

 toward the kidney. If successful the 

 mass will fill the urinary tubules and 

 also the spermatic ducts. The author 

 fixes the preparations with picro-subli- 

 mate and then hardens in alcohol, and 

 stains with alum cochineal. 



If it is desired to use a red color, then 

 carmine, 1-20, is employed instead of 

 Berlin blue, and if the veins of the kid- 

 ney are also to be injected it was shown 

 by Hyrtl that this might be done with- 

 out disturbing the urinary tubules by 

 making a hypodermic injection into the 

 tissue of the kidney. 

 (Zeitschrift fur wiss. Zoologie, Bd. 63, p. 28.) 



S. H. Gage. 



NOTES AND QUERIES. 



This space is intended for inquiries regarding 

 subjects not otherwise touched upon. Answers to 

 inquiries will be published over the signature of 

 the writer. 



" It is desired to form a permanent col- 

 lection of microscopic preparations, 

 chiefly serial sections of embryos. It is 

 desired to gain information from the ex- 

 perience of others as to what stains are 

 the least affected by the lapse of years." 



NOTICES AND REVIEWS. 



We shall be glad to notice all books, papers, re- 

 ports, bulletins, periodicals, etc., within the scope 

 of the Journal, which are sent to us for that pur- 

 pose. 



