Journal of Applied Microscopy. 



61 



Acetate of Rosanilin (Coo Hu N3 .C. H, 

 O2 ) is the most soluble rosanilin salt, 

 of large green crystals, which after some 

 time turn a brownish red. 



Test for pure magenta: It is decolor- 

 ized by sulphurous acid while impure 

 samples remain yellow or brown. 



Acid magenta, Magenta S, Rubine S 

 <Syn. Fuchsin S). — Saurefuchsin (which 

 are really impure kinds of acid magenta 

 which come on the market). Acid fuch- 

 sin is the para-rosanilinetrisulphonic 

 acid or acid salt made by trisulphonic 

 acids of pararosanilin and rosanilin, 

 through heating dried rosanilin with 

 fuming Hj SO4 at temperature of 100 to 

 170 degrees. It is about half as strong 



confuses with eosin), rubine, azaleine, 

 solferino, rubianite erythrobenzine, etc. 

 Some of the synonyms of rubin S. fuch- 

 sin are rubin S., acid magenta, saure- 

 fuchsin, acid fuchsin, fuchsin S, acid 

 rubin, acid roseine. It is generally 

 regarded that the dyes sold as acid 

 rubin, acid magenta, rubin S. rosein, 

 rose bengale (bad term as this is an 

 eosin dye, the bluest eosin known, and 

 especially useful to stain chromic acid 

 hardened material), are said to give the 

 same reactions, although different sam- 

 ples may vary somewhat in color. 

 Rosein, I believe, is the acetate of 

 rosanilin, whilst the rubin S. is the R. 

 nitrate, though by some said to be the 



Common Name. 



Rosanilin Hydrochloride. 

 Para Fuchsin 

 Rosanilin Fuchsin. 

 Syn. Acid Magenta (B). 



Fuchsin (S). 

 Acid Magenta f (M). 

 Acid Rubin. 

 Rubin S. (A). 



Acetate of Rosanilin 



or 

 Para Fuchsin. 



Scientific Name. 



Hydrochloride of 



Pararosanilin. 



Hydrochloride of 



Triamidotriphenyl- 

 carbinol. 



Acetate of 



Pararosanilin. 



Acetate of 



Triamidotriphenyl- 

 carbinol. 



Empirical Formula. 



CigHoeNaClO^. 



CjoHjsNjClO^, 

 or 

 C^oHiaNjHCl. 



Characters. 



Large glistening dark- 

 green crystals, almost 

 like Cantharides. 



CzoHigNs- C2H4O2. 

 CjaHjaNsUj. 



Large green crystals. 

 Turns to a brown color 

 when old. Sometimes 

 fxised, green, glistening 

 lumps, according to 

 preparation. 



Para l<\ichsin. 



But rarely now made 

 with acids, H2SO4, 

 HNO3. 



Rubin. 



Svn. : 

 Rosein (by B. S. S.).* 

 Fuchsin (byB. By. M. C.) 

 Magenta (by R. H.). 



Rosaniline Sulphate of 

 Para Rosanilin. 



Triamidotryphenyl- 



carbinol. 



Triamidotryphenyl- 



nitrate. 



C40H40N6SO4. 

 C20H20N4O3. 



Rosanilin Nitrate. 



^20^20^4^3* 



Very fine dark green 

 glistening powder. 



Very fine dark green 

 glistening powder. 



Acid Magenta (B). 



Syn. : 

 Fuchsin S. (B), 

 Acid Fuchsin - 

 Acid Rubin 

 Rubins. (A).t 



(M). 



Mixture of Sodium or 

 Ammon. Salts of Tri- 

 sulphonic Acids of 

 Rosanilin and Para- 

 rosanilin. 



Sodium Salts. 



C^oHisN, OioSsNa, 



Very fine powder, ma- 

 genta, bluish or green- 

 ish tint. 



in dyeing as ordinary anilin red 

 magenta. 



Test, magenta. By mixing equal parts 

 of HCl and water, magenta is decolor- 

 ized and acid magenta is unaltered, 

 though solution becomes cherry red. 



Some of the synonyms for fuchsin are 

 Toseine, anilin red (a bad term, as it 



* The capitals refer to special chemical 

 firms who designate their preparation as 

 Roseine, viz.: Brooks, Spiller, etc., of Man- 

 chester and Lincoln, England, name for 

 Rubine. 



t The acid magneta is the Rubin S. A. 

 brand, made by Actien and Anilinfabrik in 

 Bale, and Rubin S. is the dye of Chemic 

 Fabrik vom Sandoz & Co., in Basel. There- 

 fore, each dye, according to what firm 

 bought from, is liable to give a different re- 

 ■action. 



acid fuchsin (R. acetate), and certainly 

 the different dyes give different reac- 

 tions if tested on pathologic or histologic 

 material. It is of interest to remember 

 that some of the methyl derivatives of 

 rosanilin are methyl violet, methyl 

 green, etc., and the phenyl derivatives 

 of rosanilin (triphenyl-rosanilin) roson- 

 aphthylanine, cyanin, safranin, etc., and 

 as a neutral stain we have the picrate 

 of rosanilin. 



In using basic fuchsin, we find it may 

 be washed out by acid fuchsin, malach- 

 ite green, congo-roth, methyl green, 

 methylen blue, vesuvin, etc., as is often 

 seen in tubercular double staining, and 

 gives good proof of the differential 

 nature of fuchsin S., or saurefuchsin 



