Journal of Applied Microscopy. 



105 



THE FOLLOWING SCHEME MAY BE OF AID IN FOLLOWING THE METHODS HERE SUGGESTED 

 FOR STAINING PARAFFIN AND CELLOIDIN SECTIONS : 



Paraffin Sections. 



Fix paraffin sections to cleaned cover-glass 

 by floating on warm distilled water and 

 take up on cleaned cover-glass coated with 

 albumen fixative ; allow to dry. 



Celloidin Sections. 

 Place celloidin sections in distilled water. 



Heat over flame until paraffin melts. 



t 

 Wash in xylol for a few minutes to dis- 

 solve paraffin. 



t 

 Wash in absolute alcohol three minutes. 



t 

 Wash in 95% alcohol three minutes. 



t 

 Wash in distilled water thi-ee minutes. 



t 

 Stain in the hsematoxylin solution flfteen to thirty minutes. 



t 

 Rinse in distilled water. 



t 

 Wash in acid alcohol until very little 

 reddish brown stain is given off. 



t 

 Wash in tap water until sections become 



blue. 



t 



Rinse in distilled water. 



t 



Eosin solution. 



Dovible stain in 



Van Gieson's stain. 



t 



Stain about five minutes. 



t . 

 Rinse in distilled water. 



Transfer to 95 % alcohol. 



t 



t 

 Stain twenty to thirtv seconds. 



t 

 Rin.se in distilled water. 



t 

 Transfer to 95 % alcohol. 



t 



Aljsolute alcohol for five minutes. 



$ ~^ t 



Paraffin sections are cleared in oil of Celloidin sections are cleared in Carbol- 



bergamot. xylol. 



t t 



Wash in xylol. Mount on a clean slide, add a drop of Can- 



^ ada balsam, and cover with a cover-glass. 

 Mount in a drop of Canada balsam. 



Hsematoxylin and eosin stained preparations should show purplish-blue nuclei. The 

 protoplasm of cells, the red-blood cells, and all other elements of the tissue a light red hue. 



Hsematoxylin and Van Gieson's solution stained sections should show greenish blue 

 nuclei; fibrous connective tissue, red; blood cells, yellow; the protoplasm of cells, a 

 yellowish color. These stains may be used on tissues hardened after any one of the three 

 methods suggested in the first article of this series. 



{To be Continued.) 



