Journal of Applied Microscopy. 



131 



So much trouble was expeiienced in 

 filtering from the clinging- of the filter 

 to the sides of the vessel that a wire 

 basket leaving about 1 cm. space all 



Fig. 21. Hot filter for paraffin and gela- 

 tin, in section. B. B. Wire basket somewhat 

 smaller than the receptacle. The P^lannel 

 or filter paper Is put inside this. H. Closed 

 tube continuous with the water bath. The 

 Bunsen burner or the alcohol lamp is put 

 under this. 



F. Outlet of the filter. 



P. The receptacle for the paraffin, etc. 

 The cover fits inside P. and the whole Is 

 suspended by a bail. 



around was devised. The filter paper 

 or flannel is put in this and the paraffin 

 or gelatin is then poured in as usual 

 and as the filtered material oozes through 

 the sides it runs down the wires to the 

 outlet. 



For collecting with a bicycle I have 

 found a can with a very large screw top 

 very convenient. It is water tight in 

 any position, and can be easily put into 

 almost any form of bicycle carrier. A 

 leather bag attached to the handle bars 

 has proved convenient. Many times one 

 needs considerable space and a pair of 

 two-liter cans have frequently been 

 carried. 



Fig. 22. Screw-top copper can for col- 

 lecting with a bicycle. 



Fig. 23. Circulation-board for Necturi 

 and frogs. It is composed of a board 

 about 8 X 30 centimeters with a perforated 

 cork bearing a thick cover inserted in a 

 hole near one edge. B. The circulation 

 board. It is covered with cloth or blotting 

 paper. C. Sectional view showing the cov- 

 ered cork in place. 



All the metal apparatus described in 

 this article has been made of copper or 

 brass, tin rusts out too soon. For the 

 washing outfit (Fig. 18) wire netting may 

 be used, but perforated copper or brass 

 is more satisfactory, (Fig. 18 B). 



Simon Henry Gage. 



Cornell University, July 18, 1898. 



A Durable Stain for Starch. 



In vegetable histology no other re- 

 agent is used so frequently as iodine 

 either alone or in combination. Unfor- 

 tunately its effect however is transient, 

 especially where starch grains are con- 

 cerned, and consequently when perman- 

 ent preparations showing these bodies 

 stained are desired some other re- 

 agent must be employed. In the Zeit- 

 schrift fiir wissenschaftliche Mikroskopie 

 Band XIV, Heft 3. Dec. 1897, Dr. G. 

 Lagerheim has described an easy method 

 of making permanent stains of starch 

 bodies. 



The essence of the method lies in the 

 precipitation of silver in the starch 

 grains, the latter retaining a beauti- 

 ful yellowish brown to dark brown color 

 which is durable. 



The material to be colored is killed 

 with alcohol. If it contains chlorophyll, 

 the specimen is left in the alcohol until 

 decolored. 



For the latter purpose Eau de Javelle 



