170 



Journal of Applied Microscopy. 



or lost. If several slides are placed in 

 one of the Stender dishes at the same 

 time, there is always danger of hitting 

 them together and thus destroying the 

 sections. This difflculty becomes par- 

 ticularly annoying in serial sectioning, 

 where, of course, it is of the utmost 



centimeters apart by four upright stand- 

 ard pieces of the same material. These 

 upright pieces are arranged parallel to. 

 each other and at right angles to the 

 rings. Two of them extend about six 

 centimeters above the upper ring to form 

 the handle. In this way we have a 



: :;ii D 



in 



Fig. 3. 



B 



importance to preserve every section 

 intact. 



To hasten the process of staining on 

 the slide and to reduce the danger of in- 

 jury to the sections, the apparatus de- 

 scribed below has been devised. By 

 means of this device, fourteen slides can 

 be stained in the time usually required 

 for one, and the danger of injury to the 

 section is entirely obviated. This appa- 

 ratus was designed and its efficiency 

 thoroughly tested in the laboratories of 

 Cornell University. 



The apparatus was designed primarily 

 for work with Heidenhain's iron-hema- 

 toxylin, in the use of which, in order to 

 obtain a permanent stain, it is necessary 

 to wash the sections for some time in 

 running water. Hence, with the essen- 

 tial part of the apparatus, there is com- 



skeleton basket. Across the bottom ring 

 extend two parallel pieces of brass, ar- 

 ranged at right angles to the handle. In 

 the upper edge of each of these cross 

 strips are seven notches, opposite each 

 other, and of such a size as to receive, in 

 each pair of notches, the ends of two 

 slides placed back to back (Fig. I, a and 

 b). These carriers are five centimeters 

 in diameter and hold fourteen slides. 

 They are made to fit a museum jar of 

 convenient size, described above (Fig. I, 

 a). Any vessel of convenient size might 

 be used with carrier to match. 



This jar for holding the reagents is the 

 No. 2605 made by Whitall, Tatum & Co., 

 New York City. It is listed in their cata- 

 logue as museum jar — diameter two 

 inches; height to shoulder, three and 

 three-fourths inches; height to top of 



bined a washer, which will be described 

 later. The principal part of this staining 

 device is a carrier, or slide holder. It 

 consists of two rings cut out of stiff 

 sheet brass. The rings are about one- 

 third of a centimeter in width and about 

 five centimeters in diameter. They are 

 held parallel to each other and about six 



stopper, five and one-half inches; width 

 of mouth, two inches. The handle of 

 the carrier extends into the hollow 

 stopper when the vessel is closed. These 

 glass stoppers are ground to fit the 

 necks of the bottles, so that the vessels 

 are tightly closed, and in consequence 

 evaporation is prevented. 



