Journal of Applied Microscopy. 



203 



They are then stained, as used, in the 

 following manner: 



First — 1-2 min. in a 2 per cent, solution 

 of erythrosin in 67 per cent, alcohol. 



Second— 3-4 min. in Gage's Haema- 

 toxlyn. 



The result is a preparation containing 

 a large number of Leucocytes, nearly all 

 of which contain particles of carbon 

 which they have taken up from that 

 injected into the abdominal cavity. 



LIVING CILIA. 

 In demonstrating living cilia, recourse 

 is usually taken to the amphibians or 

 some other of the lower vertebrates. The 

 reason is that it has long been a current 

 theory that the cilia of cold-blooded ani- 

 mals live longer than those of mammalia 

 after the death of the animal, and hence 

 afforded better opportunity for observa- 

 tion: This does not prove altogether 

 true. During the past month ciliated 

 epithelium was taken from the trachea 

 of a young cat. It was placed immedi- 

 ately in some of the clear liquid obtained 

 from puncturing the eyeball, covered, 

 and sealed with castor oil. In a few 

 moments rapid motion of the cilia could 

 be distinguished. This motion rarely 

 ceased before eight hours had elapsed. 

 A few preparations retained their activ- 

 ity for fifty-six hours. This is as long as 

 amphibian cilia continue to move. Thus 

 it seems that mammalian ciliated epethe- 

 lium offers as good material as amphi- 

 bian for the purpose of showing the 

 action of living cilia. 



C. M. M. 



St. Louis Microscopial Society met Oc- 

 tober 15th, and after transaction of usual 

 business proceeded to the scientifis pro- 

 gramme. 



Dr. Bremer reported a doubtful ulcer 

 of the tongue, examined by himself, to be 

 a syphaloma undergoing degeneration. 

 Excision of the tongue was recommended 

 as a means of relieving the pain caused 

 by tissue changes affecting sensitive 

 nerves. 



Dr. Alt read a paper on Adenoma of the 

 ciliary body, in four cases he examined, 

 the finer structure of the tumor 

 resembled a gland whose efferent duct 

 had been obstructed, the epithelium had 

 sprung from that of the pars ciliaris 

 retanae. None of these tumors awak- 

 ened the thought of malignancy. In no 

 case had blindness nor pain been caused, 

 nor did any of these tumors resemble 

 anything hitherto described. 



The society met again on October 27th, 

 and after the customary business routine 

 Dr. Crandall presented the kidneys and 

 bladder of a patient who had died after 

 suffering two years from cystitis. From 

 the left ureter. Dr. Crandall had made 



pure cultures of bacterium coli 

 communis. 



Dr. Johnson presented the lungs of a 

 child who had died at the age of three 

 months as a result of pulmonary hemorr- 

 hage. The case was presented as one of 

 congenital tuberculosis following bladder 

 tuberculosis in the mother, in whose 

 urine the tubercule bacilli ha:l been 

 found. W. B. 



Several years ago I accidentally left 

 some material of Aster Novae Angliae 

 in the paraffin bath at 52 degrees C. for 

 nearly a week. I supposed of course that 

 the material would be ruined, but found 

 that it was in very fair condition. Since 

 that time I have frequently left material 

 in the bath for several days, and some- 

 times found it in really fine condition. 

 Material forgotten by pupils has also 

 proved to be in fine condition after such 

 a prolonged bath. We have never had 

 confidence enough in such prolonged 

 treatment to try it intentionally with 

 material known to be well killed and 

 fixed. 



We seldom keep material in the bath 

 for more than two hours, and delicate 

 tissues are thoroughly infiltrated in much 

 less time. It may be possible that tis- 

 sues which show shrinkage or plasmoly- 

 sis after an hour's bath may regain their 

 normal form if the bath be prolonged for 

 days. I should be glad to hear the ex- 

 perience of other workers. C. J. C. 



In the Botanical laboratory of the Uni- 

 versity of Nebraska we have been using, 

 for some years, a convenient reagent 

 bottle which appears not to be in general 

 use, and which has commended itself to 



us above all others which we have tried. 

 It consists of a rather broad base (50 

 mm.) with a wide mouth (20 to 22 mm.) 

 into which is fitted a hollow glass stopper 

 as shown in the accompanying figure. 



