1632 Journal of Applied Microscopy 



In conclusion he regards the formation of the nodules and milk patches as 

 identical pathological processes, and considers their etiology as referable to 

 mechanical causes from high and irregular blood pressure producing chronic 

 irritation of the epicardial surface, especially on exposed parts. For neither 

 does he support the theory of an origin from a localized pericarditis nor from 

 developmental irregularities in the epicardium. H. A. Christian. 



• M «7 ^- r ■ -.r •■ J The author found the " formal-methyl " 



Murawieif, W. Die femeren Verandeningen ■' 



durchschnittener Nervenfasern im periphe- method of great service in the Study of 

 ren Abschnitt. Ziegler's Beitrage ziir path, ^oth normal and pathological nerve 

 Anat. 29: 103, 1901. \ ° 



fibers. It was devised several years 



ago by Rossolino and Murawieff. 



The fresh nerve is placed in a four per cent, formalin solution, where it 

 should remain not less than two or three days. It is then transferred to 95 

 per cent, alcohol for twenty-four to forty-eight hours, but never longer than 

 forty-eight hours. Tease out a small piece of the nerve, thus hardened, and 

 stain for ten to fifteen minutes in a one-half per cent, aqueous solution of methy- 

 len blue which is heated until the first bubbles appear. Cool the solution and 

 move the tissue into anilin oil for one-half a second (anilin oil one part, 95 per 

 cent, alcohol nine parts), then into 95 per cent, alcohol for one-half a second. 

 Clear in cajeput oil. Place the bits of nerve on a slide, add a drop of Canada 

 balsam. Tease the nerve fibers thoroughly and cover the preparation with a 

 cover-slip. 



If one wishes to make a cross section of the nerve, after hardening in four 

 per cent, formalin, place it in 95 per cent, alcohol twenty-four hours, then in abso- 

 lute alcohol twenty-four hours. Embed in celloidin. Stain in the manner already 

 described. j. h. p. 



CURRENT BACTERIOLOGICAL LITERATURE. 



H. W. CONN, Wesleyan University. 



Separates of Papers and Books on Bacteriology should be Sent for Review to H. W. Conn, 

 Wesleyan University, Middletown, Conn. 



Chester. A Manual of Determinative Bacte- Prof. Chester, of the Delaware College 



riology. Macmillan& Co.,p. 400. Price$2.6o. a • 1^ 1 t- ■ ^ oi. ^- u 



^■' ^ ^ Agricultural Experiment Station, has 



done great service to bacteriologists in the publication of this work. He attempts 

 to give a brief deterniinative description of all species of bacteria which have been 

 sufficiently described for the purpose. Two preliminary chapters briefly describe 

 methods of characterizing species. In these chapters he eliminates long de- 

 scriptive phrases and puts in their places short characteristic terms, which 

 very greatly reduce the number of words. The bulk of the work is a compila- 

 tion of bacteria descriptions. The groups are divided according to Migula's 

 recent classification and, by the use of analytical keys and family synopses, it is 

 made easy for one to determine rapidly where a given bacterium should be clas- 

 sified. The work does not pretend to contain a full, systematic description of 



