1670 Journal of Applied Microscopy 



ology which are opened by the new chemistry, and deprecates the fact that the 

 discussion of so many of the problems must now be purely theoretical, while 

 experimentation along the lines indicated by the comparatively small amount 

 of work which has been done in applying physico-chemical laws and methods 

 to physiological problems, would place them on a sound basis of fact. While a 

 considerable portion of Brasch's work is necessarily theoretical, from the very 

 fact that so little experimental work has been done on the subject, it will 

 nevertheless be found very suggestive and helpful both to the teacher and the 

 investigator. r. p. 



Popielski, L. Ueberdasperipherischereflecto- The author finds that the pancreas can 



rische Nervencentrum des Pankreas. Arch. function after complete separation from 

 f. d. ges. Physiol. 86: 215-246, 1901. ^ ^ 



the central nervous system, this reflex 



activity being mediated by local nerve centers in the organ itself. He concludes 

 that the central nervous system functions in unifying and regulating the inde- 

 pendent activity of the different organs of the body. r. p. 



Oswald, A. Ueber die chemische Beschaffen- A technical chemical study of the 



heit und die Function der Schilddruse. thyroid with a brief discussion of its 



Strassbarg (Karl J. Trubner), 1900. P. 61. ■' 



(Price iMk. 50 Pf.). functional importance and method of 



activity. R. p. 



NORMAL AND PATHOLOGICAL HISTOLOGY. 



JOSEPH H. PRATT, Harvard University Medical School. 



Books for Review and Separates of Papers on these Subjects should be Sent to Joseph H. Pratt, 

 Harvard University Medical School, Boston, Mass. 



Wright, J. H. Eine schnelle Methode zur dau- The rapid method devised by Wright 

 ernden Aufbewahrung gefrorener Schnitte. of making permanent mounts of frozen 

 Cent. f. allg. Path. u. path. Anat. 12: 634, ..... , , 



190 1. Also Mallory and Wright, Pathologi- sections IS a distinct advance over the 

 cal Techique, Second Edition, p. 417. Phila. procedure advocated by Cullen. Work- 

 ing with a good microtome and a sharp 



knife, Wright and his pupils have made preparations fully equal to paraffin or 



celloidin sections. 



1. Place the specimen in a ten per cent, solution of formalin for two hours 

 or more. If an immediate diagnosis is desired, the specimen may be boiled in 

 the same solution, in a test tube, for two or three minutes. Naturally, the slower 

 method gives better histological detail. The bit of tissue should not measure 

 over five mm. in thickness. 



2. Wash in water. 



3. Cut frozen sections. The carbon-dioxide freezing microtome is the most 

 satisfactory. The blade of a carpenter's plane mounted in a wooden handle 

 makes an excellent microtome knife. The sections should be as thin as possi- 

 ble. In order to obtain sufficiently thin sections the knife must be very sharp. 

 It should be given a smooth edge by rubbing on a razor strop. 



