1688 



Journal of Applied Microscopy 



entirely practical to collect the blood, send it to the laboratory," and have a 

 report on the following day. 



THE EXAMINATION OF WATER FOR TYPHOID FEVER BACILLI. 



Typhoid fever is considered an infectious disease, the source of infection 

 being probably almost entirely in drinking water. It is necessary, therefore, 

 that a bacteriological examination of the drinking water should be made when 

 the disease is prevalent. The following preliminary preparation is necessary: 



TO STERILIZE GLASS WARE. 



Wash thoroughly one 500 c.c. Erlenmeyer flask, twenty-four test tubes, size 

 15 X 150 millimeters. Drain out the water and plug with cotton. Wash six 

 100 c.c. glass stoppered bottles, six 1 c.c. pipettes, wrap the bottles and pipettes 

 each one separately in plain white paper and tie with a string. Place all in a 

 hot air sterilizer. Gradually heat the sterilizer up to 1")0°C. for an hour or 

 longer, until the cotton plugs and the paper are slightly charred. Allow the 

 glassware to cool in the sterilizer, after which remove and store it in a drawer 

 till needed. 



M.=^.^ 



Erlenmeyer Flask. 



Graduated Pipette. 



Physician's Hot Air Sieiilizer. 



Prejtare nutrient bouillon as follows: 

 Distilled water, - 

 Extract of meat (Liebig's), 

 Dry peptone (Wittes), 

 Sodium chloride (common salt). 

 Grape sugar (or common sugar). 



500 c. c. 

 2.5 grams. 

 15 grams. 

 2.5 grams. 

 2.5 grams. 



Mix and neutralize with a saturated solution of sodium carbonate, added 

 drop by drop to the bouillon until it gives a very slight tinge of blue to neutral 

 or red litmus paper. The bouillon should be boiled for fifteen or twenty min- 

 utes and filtered. If not clear, it is either too alkaline or there is a precipitate 

 of albuminates. Test again for alkalinity, adding a little dilute acetic acid if 



