and Laboratory Methods. 1705 



CYTOLOGY, EMBRYOLOGY, 



AND 



MICROSCOPICAL METHODS. 



AGNES M. CLAYPOLE, Throop Polytechnic Institute. 



Separates of Papers and Boolis on Animal Biology should be sent for Review to Agnes M. Claypole, 



55 S. Marengo Avenue, Pasadena, Cal. 



h 



Spuler, A. Ueber eine neue Stueckfaerbe- The author uses finely powdered 

 methode. Deutsche med. Wochenschr. 27 : cochineal boiled in distilled water, 

 ' ^ ■ filtered and not entirely dried. Then 



distilled water is added and the mixture filtered. Pieces of tissue are put into 

 this solution for twenty-four hours or longer, in a warm place on the paraffin 

 oven. They are then washed off and soaked for a long time in a bath of iron- 

 alum, which quickly changes the color from red to black. After this treatment 

 the preparations are thoroughly washed in distilled water and imbedded in the 

 usual way. Iron-alum, tannin or any other mordant may be used simultaneously 

 with the stain. 



This new method gives homogeneously stained bulk material, preparations 

 from which prove highly satisfactory for projection purposes ; since they have 

 an intense black color. The cell contours are as clear in outline as the nuclei, 

 so that the preparation gives the impression of a pen drawing. a. m. c. 



Willebrand, E. A. V. Eine Methode fur gleich- The author experimented with combina- 

 zeitige Combinationsfaerbuna; von Blutt- . ^ • i i i i i • 



rocken praeparaten mit Eosin und Methylen- tions of eosm and methylen blue in 

 blau. Deutsche med. Wochenschr. 27: 59, both acid and alkaline solutions. He 



used a liquid mixed with acetic acid 

 containing methylen blue in excess, which gave most satisfactory results ; five 

 per cent, eosin was dissolved in seventy per cent, alcohol, to which was added 

 equal parts of concentrated aqueous solution of methylen blue. Used in this 

 form the stain gives preparations colored a diffuse blue. Adding about ten to 

 fifteen drops of a one per cent, solution of acetic acid to about 50 c. c. of the 

 stain, a very different result is obtained. If the blood preparations are well fixed 

 in dry heat, absolute alcohol, or one per cent, formol-alcohol, and stained for 

 five to ten minutes with repeated warming and subsequent washing, with no 

 decolorization, the following result is obtained : Erythrocytes are red, nuclei 

 dark blue, neutrophile granules violet, acidophile red, and the granules of the 

 mast cells are intense blue. a. m. c. 



Heinz, R. Ueber Blutdegeneration und Regen- ^one marrow concerned in regenera- 

 eration. Beitr. z. pathol. Anat. u. z. allgem. tion shows a very complex Structure. 



Pathol. 29: 200-404, ^ Tfln., igoi. ^, . ._ r . 1 • 1 1 ^ 



yv t t> J , y -pj^g significance of the single elements 



depends in large measure on the histological technique of the method used. 

 Staining methods are extremely well worked out. There are in most cases sev- 

 eral staining and fixing methods available. For example, there are many hema- 



