and Laboratory Methods. 1731 



in the air. Mount film side down in a drop of balsam on a clean slip. The 

 bacillus of tetanus will appear like pins, the spore at one end of the bacillus 

 resembling a pin head. 



Fig. IX. — Bacillus of tetanus. Culture stained with Fig. X. — Bacillus anthrax, from blood. Stained 

 methyl-violet. Magnified 1200 diameters ; y'^-inch with Loeffler's alkaline methylen-blue. Magni- 

 oil immersion objective, Bausch & Lomb compen- fied Soo diameters; Js-inch objective, Zeiss pro- 

 sating photo ocular No. 2. jection ocular No. 4. 



BACILLUS ANTHRACIS— Splenic Fever. 



The blood of animals suffering from splenic fever always contains the 

 bacillus of anthrax. To prepare a slide for examination, a drop of blood should 

 be drawn from a thoroughly cleansed surface, preferably the ear, which should 

 be washed with soap and water, then with alcohol, and finally with ether. A 

 blood lancet should be used, as it insures a free flow of the blood. Cleanse two 

 glass slips with alcohol first, then with ether. Apply one to the blood drop so 

 that a h?lf-size drop may adhere to it within about one-half inch of one end. 

 Apply the end of the other slip to the blood so that the edge rests lightly in 

 the blood and in contact with the glass beneath. Draw this slip, which acts as 

 a spreader, gently along the first or blood slip, making a thin spread two-thirds its 

 length. After the film of blood has dried in the air the slip with the film side 

 up should be heated over an alcohol flame or Bunsen burner until it is too hot 

 to hold in the fingers. Cool it partially and reheat. Stain the film with Loeffler's 

 alkaline-blue, which should be liberally applied. Wash in water and dry with 

 filter paper. Put a drop of balsam about the center of the slip, which will be 

 about the center and best part of the f^lm ; a clean cover will complete and make 

 a permanent preparation. This method yields good slides of the blood as well 

 as of the bacilli, showing the pathological changes that may take place in the 

 blood and frequently white corpuscles engulfing the bacilli. The bacilli appear 

 as short rods with square ends, which are a trifle thicker than the body. The 

 ends also appear indented. 



ASIATIC CHOLERA. 



The bacillus of Asiatic cholera is found in the evacuations from the intestinal 

 canal, and in most cases the disease may be recognized from preparations made 

 direct from the evacuations as follows : Take a small portion of one of the slimy 



