1732 



Journal of Applied Microscopy 



flakes in the stool, spreading it with the platinum loop on a clean cover-glass 

 held in a Cornet forceps. Dry in the air and pass rapidly through the flame of 

 an alcohol or Bunsen burner three times. Apply a quantity of carbol fuchsin 

 for about one minute. Wash in water, dry, and mount film side down in a drop 

 of balsam on a clean slip. Stain another cover with Loeftler's alkaline-blue, and 

 another with gentian-violet. If upon microscopical examination only curved 

 rods are seen, their long axes extending in the same direction, the diagnosis of 

 Asiatic cholera will be reasonably certain. If only a very few such rods are 

 seen, a portion of the stool should be sent to the state or municipal laboratory 

 with a history of the case for a bacteriological examination. A positive opinion 

 is possible within twenty-four to thirty-six hours. 



Fk;. XI.— Batillu.s A-iatie Lh.ilcia. Stools. Stained 

 with Loeffler's alkaline methylen-blue. Magnified 

 )2oo diameters; j'j-incli oil immersion objective. 

 Kausch i"t Lomb compensating ociilar \o. i. 



Fii.. XII.— Bacillus mallei. ( Uandcrs, from nodule. 

 Stained with gentian-violet. Magnified 1200 di- 

 ameters ; ,V-inch oil immersion objective, Zeiss 

 |)rojectioi\ ocular No. 4. 



MALLEI— Bacillus of Glanders. 



This disease, being a rather common one among horses and on account of 

 its transmissibility to man and its not infrequent fatal termination, becomes an 

 interesting study to the medical man. In horses the bacilli are found in the so- 

 called glanders nodules in or upon the mucus membrane of the nose about the 

 larynx, and not infrequently in the lungs. Deep ulcers develop from these 

 nodules in the nose, especially about the turbinated bones. Microscopical ex- 

 aminations should be made from the ulcers and from the nodules, the latter 

 being opened for this purpose. With the platinum loop take up some of the 

 contents of a nodule and spread it on a clean cover-glass held in a Cornet for- 

 ceps. Dry in the air, pass three times, film side up, through the flame of an 

 alcohol lamp or Bunsen burner. Stain from one to five minutes with carbol 

 fuchsin, methyl-violet, or Loefitler's alkaline methylen-blue. Wash in water, dry 

 between filter paper, and mount, film side down, in a drop of balsam on a clean 

 slip. The bacilli will appear as rods with rounded or slightly pointed ends. 

 The bacilli stain irregularly, containing dark and light areas without any 

 regularity whatever. The light spots are not spores. 



In man the disease usually occurs in those handling diseased horses. It 



