1752 



Journal of Applied Microscopy 



Fig. 81. 



of pressure within, due to the absorption of the oxygen in the apparatus, causes 

 the over-pressure from without to force the cover tightly over the saucer, form- 

 ing a hermetical seal. In order to reopen 

 the apparatus the cover must be turned before 

 it can be taken off. 



Tramhusti's Method. — The apparatus used 

 by this investigator is illustrated in Fig. 31. 

 It consists of two parts : a cone-shaped flask 



(A) containing the medium, and a cylinder 



(B) which is screwed into (A) by means of a 

 fine thread. In the interior of (B) there is a 

 small tube which opens into (A). 



Alethod. — Pour the inoculated medium, 

 gelatin or agar, into flask (A) and distribute 

 it over the surface of the bottom uniformly, 

 the same as in case of a petri dish. Screw 

 (B) into (A), remove the stopper and fill (B) 

 with an alkaline solution of pyrogallol (2 grams 

 of dry pyrogallic acid plus 15 c. c. of 10 per 

 cent, solution of KOH), care being taken that 

 the reagent does not enter the small tube. Replace the stopper which has 

 previously been covered with paraffin or vaseline and remove the apparatus to 

 the incubator. 



Arens in 1894 used a common small desiccator with a ground glass cover for 

 anaerobic plate cultures. 



Method. — Fill the desiccator partly with quartz sand, to this add an optional 

 amount of dry pyrogallic acid, leaving just room for one 

 or two small petri dishes. Use deep layer of agar in the 

 plates. Before putting the plates into the apparatus pour 

 a considerable amount of a 10 per cent, solution of caustic 

 potash all over the surface of the sand and acid. Then 

 place the petri dish, minus cover, upon the sand and close 

 the desiccator with a well vaselined ground glass cover by 

 rotary motion. 



Lubinsky in 1894 modified Buchner's method for tube 

 cultures. Instead of putting the culture tube into a large 

 test tube, Lubinsky placed it in a glass cylinder (Fig. 32) 

 12-15 cm. high and 3-4 cm. in diameter. Into this cylinder 

 he pours the alkaline solution of pyrogallic acid ; then he 

 pushes a snugly fitting, manifold perforated cork about half 

 way down into the cylinder. In the center of the cork there 

 is a large perforation in which the culture tube is inserted. 

 The cylinder is closed with a glass stopper of ground glass and the edges 

 are sealed with paraffin. When sealed he shakes the apparatus vigorously for 

 2 to 3 minutes to hasten the process of absorption. 



Fig. 32. 



