1764 Journal of Applied Microscopy 



CURRENT ZOOLOGICAL LITERATURE. 



CHARLES A, KOFOID, University of California. 



Books and Separates of Papers on Zoological Subjects should be Sent for Review to Charles A. 

 Kofoid, University of California, Berkeley, California. 



Argutinsky, P. Malariastudien. Arch. f. Mik. Blood of malarial patient is spread upon 

 Anat. 59: 315-354, Taf. 18-21, 1901. .^u vj i. t j t> -u > 



^ -^ "^-^ ^ the slide by Janczo and Rosenberger's 



method, dried at the room temperature and at once placed in sublimate-alcohol 

 prepared as follows : Seven grains of pure sublimate are dissolved in 100 cm.^ of 

 one per cent, hot aqueous solution of common salt, and when cool 100 cm.-^ of 

 absolute alcohol are added. This faxing fluid is allowed to act for 5 to 8 minutes 

 and the slides are then washed in strong alcohol for a few minutes, and 

 transferred to iodine alcohol (100 cm.'^ absolute alcohol -f 2cm.-^ of alcoholic 

 tincture of iodine). This tincture is prepared by adding 1 gram of pure iodine 

 to 100 cm.^ of absolute alcohol. After rinsing in pure absolute alcohol and dry- 

 ing between filter paper, the slides are ready for the stain. The soda-methylen 

 blue and eosin stain was used, prepared from methylenblau medicinale Hochst 

 and eosin B A extra Hochst. Two methods now were employed. (1) Very 

 dilute solutions without subsequent differentation. Two stock solutions are 

 are made up : (a) J^ per cent, eosin ; (b) soda-methylen blue, prepared by heating 

 for 48 hours in a warm oven (55-60°C.) lOOcm-^ of 1 per cent, methylen blue 

 with 6 cm.^ of 5 per cent, solution of soda. This can be used immediately, and 

 remains in good condition for many days. This stain when ripened at room 

 temperatures may be used undiluted, but is not so well adapted for staining in 

 dilute solutions. Contrary to the recommendations of other malaria technicians, 

 our author finds that warming the fluids during staining is unnecessary and tends 

 to cause precipitates. Neither is it necessary to vary the proportions of the eosin 

 and the soda-methylen blue or to use different dilutions in order to secure 

 , results with different stages of the parasite, even the most difficult. Precipitates 

 are avoided by more dilute solution and by decreasing the proportion of eosin. 

 The following is the author's procedure : Three cm.^ of the soda-methylen blue 

 solution prepared as above directed is mixed with 42 cm. -^ of distilled water. In 

 another dish 5 cm.^ of the J^ per cent, solution of eosin is mixed with 25 cm.^ of 

 distilled water and the two mixtures are then slowly stirred together. Slides are 

 placed in the stains for 15 to 20 minutes. The metallic film which forms on the 

 surface of the stain is removed with filter paper before the slides are taken from 

 the staining dish. The preparations are washed one to two minutes in a series of 

 dishes of distilled water, and after drying between filters are mounted in balsam. 

 (2) With undiluted solutions of 1 per cent, soda-methylen blue and 1 per 

 cent, eosin, with subsequent differentation. The stock solutions for this method 

 are 1 per cent, eosin and the soda-methylen blue mixture as in (1), ripened at 

 room temperatures. After 5 days the stain may be used, and it remains in good 

 condition for several weeks. To 15 cm.^ of the 1 per cent, soda-methylen blue 



