1766 Journal of Applied Microscopy 



Heidenhain's iron-haematoxylin following Hermann's fluid, and used either alone 

 or in combination with rubin or ruthenium red. c. a. k. 



Needham, J. G. and Hart, C. A. The Dragon- Formalin is not a good fixing agent for 

 files I Ot/o/iata)oi IWinois; Fart I, Pe^aiur/da;, ... ^ c i • u -., 



AescLw.^ and Gomj>/aWc^. Bull. 111. State aquatic insects of large Size, such as 

 Lab. Nat. Hist. 6: 1-94, pi. i, 1901. the nymphs of the Odonata. Hot alcohol 



penetrates more readily, preserving internal structures from decomposition. It 

 also serves to preserve to some extent the fugitive colors of the imago. The 

 nymphs may be secured by examining their haunts in ponds and streams with a 

 D-shaped dip-net. The images are most readily collected early in the morning. 

 The nymphs creep up upon sticks, vegetation, piling, or boats, and the imago 

 emerges from the larval skin. When fully dried and expanded the images thus 

 secured afford the finest of specimens. Endophytic oviposition occurs among 

 the Zygoptera and Aeschnidc^, the eggs being placed by the female in the stems 

 or leaves of aquatic vegetation. The Gomphidce, Cordnlegasterida, and Lihellulidm 

 deposit their eggs free in the water. Eggs of Gomphus are easily secured in the 

 laboratory by holding an ovipositing female with the fore wings back to back 

 and repeatedly touching the tip of the abdomen to a dish of water. Oviposition 

 in captivity was not secured from other genera. c. a. k. 



Rottmano, 0. Ueber del Embryonalentwick- -pj^g ^f IqH^o, Sepia, Octopus and 



lung der Radula bei den Mollusken. I. Die °° , . 



Entwicklung der Radula bei den Cephalopo- Elodone were fixed m sublimate or 

 den. Zeitschr. f. wiss. Zool.70: 236-262; chrom-osmo-acetic of Flemming. To 

 Taf. XI, XII, 1901. , „ . 



avoid the shattermg of the yolk in 



sectioning after long exposure to clearing fluids or the paraffin bath, the follow- 

 ing procedure was adopted : Absolute alcohol, 24 hrs. ; xylol, 2 hrs. ; solution 

 of paraffin in xylol in warm oven, ^2 hr. ; pure paraffin, 1 hr. Eggs thus treated 

 were fully infiltrated and cut satisfactorily. For the demonstration of the earliest 

 stages of the developing radula, sections previously stained in Heidenhain's 

 iron-htematoxylin were further stained in a solution of Bismarck brown in abso- 

 lute alcohol for a few minutes ; the excess of stain was then washed out in 

 absolute alcohol, and the preparations cleared in xylol, and mounted in balsam. 

 The youngest radula is stained an intense yellowish brown, and the precision 

 of the staining of the preparation in general enhanced by this process. 



c. A. K. 



Certes.A. Colorabilitc elective, "intra vitam," Sporiferous filaments of Spirobacilhis 



des filaments sporiferes du Spirobacillus ^^^ ^f j^^^y Other bacteria are Stained 

 gigas (Cert.) et de divers microorganismes 



d'eau douce et d'eau de mer par certaines " intra vitam " by methylen blue. That 



couleurs d'aniline. Compt. Rend. Assoc. q{ Qriibler and of Hochst is most pre- 

 Franc. Av. Sci. 1901 ; 9 pp. 3 pi. 1901. 



cise, but also the most toxic. Bleu 

 d'Ehrlich was the most readily used and gave the best results. Various Proto- 

 zoa, such 2iS- Amcvba, Parama'cium and other ciliates, and the suctorian Sphctro- 

 phrya, are also affected while living by some aniline stains, but with less cer- 

 tainty. The nucleus of Amoeba is stained by acid green, or by dahlia. Mala- 

 chite green stains both nucleus and the cytoplasm of Chi/odon, Lacrytnaria, 

 Spirostomum, and Spharophrya, and the cytoplasm of Stentor and Parama'cium. 

 In conjugation stages of the latter, however, the fragmented nucleus takes the 

 stain. The peduncle of Vorticella is colored by Bleu C 2 B de Poirrer (1886), 

 though not by the more recent products of this manufacturer. Diphenylamine 

 blue, methyl blue, methylen blue and Congo red did not yield satisfactory results 

 except in the study of food vacuoles. c. a. k. 



