1808 



Journal of Applied Microscopy 



for liquid culture media belonging to the same fermentation tube principle. 

 Being of large capacity they are especially adapted for the cultivation, on a large 

 scale, of the tetanus bacillus for the production of a tetanus toxin. 



In apparatus illustrated in Fig. 51 there are two bulbs (A) and (B) of nearly 

 equal capacity, connected with a heavy rubber tube (C) which carries a clamp (D) 

 to regulate the communication between them. This apparatus is best manipu- 

 lated in a tin rack (F). The bouillon occupies the whole of (A) and all below 

 the dotted line in (B). It is inoculated by transferring the culture material with 

 a platinum loop or pipette through the cotton plugged opening (E). The growth 

 travels down into bulb (A) within 24 hours. 



Fig. 52 represents an apparatus consisting of a stout liter flask (A), into 



Fig. ^^2. 



Fig. 53. 



which is fitted a rubber stopper (c) carrying a 100 c. c. pipette (b) with the lower 

 portion bent as shown in Fig. 52, and the upper shortened and provided with a 

 cotton plug. The bouillon fills the flask completely and extends down the nar- 

 row tube to the dotted line in the bulb. The inoculation takes place through the 

 opening (d), the growth proceeds unaided along the narrow tube and reaches the 

 flask in from 24 to 36 hours. 



The second form (Fig. 52) cannot be autoclaved when filled, as some of the 

 fluid will be thrown out. To obviate this the flask is only partly filled and the 

 extra bouillon required is autoclaved with it in an ordinary flask. There is no 

 difficulty if the Arnold sterilizer is used. 



