and Laboratory Methods. 1827 



[ NEWS AND NOTES. j 



The eighth annual session of the Biological Station of the University of 

 Indiana will be held at Winona Lake, Kosciusko county, Indiana, between June 

 23 and August 22. The station is a field laboratory and especial emphasis is 

 laid on field work. In general the work will be adapted to the needs of medical 

 preparatory students and of teachers and investigators in the lines of Zoology, 

 Physiology, Botany and Nature Study. 



The Harpswell Laboratory of Tufts College located at South Harpswell, on 

 Casco Bay, which is sixteen miles from Portland, Maine, will be open from June 

 16 to September 13, 1902; the regular courses of instruction beginning July 2 

 and continuing for six weeks. Courses will be given in Intermediate Zoology, 

 Vertebrate Zoology, Botany and Embryology. The laboratory also provides for 

 opportunities for original research. Communications concerning the laboratory 

 should be addressed to the director, J. S. Kingsley, Tufts College, Mass. 



The University of Montana Biological Station will open July 14, and continue 

 five weeks, or until August 16. Courses in Zoology, Botany and Nature Study will 

 be given and ample opportunity for original work in the unexplored regions sur- 

 rounding the station. Information concerning the station may be obtained from 

 Morton J. Elrod, Missoula, Montana. 



The eleventh session of the Hopkins Seaside Laboratory of Leland Stanford 

 Jr. University will begin June 9, 1902, and continue six weeks, closing July 19. 

 The laboratory provides for three classes of students : Teachers and students, 

 for whom regular laboratory courses in Botany and Zoology are arranged ; ad- 

 vanced students in Zoology, Physiology, and Botany ; and investigators who are 

 prepared to carry on researches in Morphology and Physiology. 



Methods in the Culture of Unicellular Alg^. — Knop's solution either 

 as a fluid culture or in connection with agar (5 to 7 grams to the liter of nutrient 

 solution) gives very satisfactory results. Stender dishes serve well for culture 

 vessels, as it is desirable to have as large an amount of the medium as possible 

 and also considerable surface for growth. Sterilization must be as complete as 

 in bacteriological work. It is found better to deposit the algal cells on the moist- 

 ure that collects upon the surface of the agar after sterilization than to place 

 them directly upon the agar, as in the latter case growth is retarded ; but when 

 deposited in the moisture, which slowly evaporates, the contact with the agar is 

 gradual and growth proceeds normally. 



When it is desired to make direct and frequent examination of cultures a very 

 satisfactory method is to isolate a single cell on a slide in sterilized water or 

 nutrient solution and place over it a cover-glass supported by wax feet. Evap- 



