1880 Journal of Applied Microscopy 



second for the date of inoculation, the third indicates the medium^ upon which 

 the culture is grown, while the fourth is for short notes and references. 



Animals are regarded as one form of culture medium. Cultures made from 

 different organs of the same animal are regarded as sister cultures. The name 

 of the organ from which each individual culture came is entered in the column 

 under " Remarks." 



In cases where only nine or ten inoculations of media are necessary, the 

 first card will contain the entire history of the work done. An extension card, 

 printed on both sides with the same form as that given on the back of card I, 

 may be used when more than this number of cultures is to be recorded. 



401.11 



XI-10-01. — A thin, slimy, transparent, amber growth. 



XI-15-01. — Color now dark brown. 

 Growth opaque. 



Card II. 



Card II. Supplementary Card. — In any case where the results of an 

 inoculation are too extensive to be satisfactorily stated on the culture record 

 card, a blank card is used for this purpose. Such a card is identified by the 

 number of the culture which it describes. A note referring to this card should 

 be made in the " Remarks " column of the culture record card. 



The culture record, extension, and supplementary cards are filed together. 



5 A system of abbreviations and initials is used to indicate the media on wliich the culture 

 is grown, thus : 



G. gelatin. G. P. guinea pig. 



A. agar. G. A. glycerine agar. 



M. milk. P. potato. 



S. serum. 

 A record is kept of the details of making of each lot of media, etc. (see Media card). 

 Each lot of each kind of media receives a number at the time it is made. These numbers, 

 with the abbreviations above given (e.g., GA 13; A 12; etc.), serve to positively identify each 

 tube of media. 



