1938 Journal of Applied Microscopy 



shorten themselves as fusion progresses. The second sperm attaches itself 

 to the upper polar nucleus ; these two are soon joined by the lower polar nucleus, 

 and the three fuse simultaneously. The endosperm nucleus divides before the 

 fertilized egg shows signs of division. This is true of all forms except Naias, in 

 which, according to Guignard, the egg divides first. The two deeply staining 

 bodies observed by the reviewer in the pollen tubes of Silphium and Erigeron 

 were also seen in Monotropa. Their origin was not determined. The article 

 is illustrated with ten excellent figures. W. J. G. Land. 



Chicago. 



Ducamp, L. Recherches surTembryogenie des In the Araliacese, as a rule, there is 

 Araiiacees. Ann Sci. Nat. Bot. Ser. VIII. ^^^ ^^e archesporial cell in the nucel- 

 15: 311-402, pis. 6-13, 1902. ■' ^ 



lus of the ovule. This cell gives rise 



to a tapetal cell and a sporogenous cell which is the megaspore mother cell. 

 The megaspore mother cell may develop directly into the embryo-sac or may 

 give rise to an axial series of three or four potential megaspores. In Fatsia 

 Japonica a case is figured in which the middle cell of an axial row of three cells 

 had divided longitudinally. In another case the mother cell had divided trans- 

 versely and each daughter cell had then divided longitudinally. Similar divis- 

 ions seem to occur in Aralia racetnosa. In one case the mother cell is described 

 as having given rise to an axial row of four cells, the upper three of which had 

 subsequently divided longitudinally. The writer states that the division of 

 the nucleus of the mother cell sometimes takes place without the formation of 

 cell walls, but the figures are very small and may bear another interpretation. 

 The megaspore nearest the chalaza usually functions, but the middle one in the 

 axial row develops at the expense of those above and below. Double fertiliza- 

 tion was looked for, but nothing definite was found. The development of the 

 embryo and appearance of tissue systems are described in detail. c. j. c. 



CYTOLOGY, EMBRYOLOGY, 



AND 



MICROSCOPICAL METHODS. 



AGNES M. CLAYPOLE, Throop Polytechnic Institute. 



Separates of Papers and Books on Animal Biology should be sent for Review to Agnes M. Claypole, 



55 S. Marengo Avenue, Pasadena, Cal. 



Bogdanow, N. O proisschoshdenii i snatsch- ^^^"^ dried smear preparations of blood, 



eniieosinofilnoisernisstosstiiobotnoschenii sputum, pus, etc., are fixed in equal 

 eja k prozessy krowetworeniia Tueber die ^ r , 11,1 /xt-i -r , 



Entstehungund Bedeutungder eosinophilen P^rtS of ether and alcohol (Nlklforow S 



Kornung und ihre Bedeutung fiir die Blut- mixture) for 20-30 minutes, then 

 bildung.] Mang. Diss. Moskwa 188 pp., ^ • 1 r o c • . • 1 



Tafln 2, 1899. stamed for 3-5 minutes in a 1 per 



cent, solution of eosin in 60 per cent, 

 alcohol which has been diluted one-half with distilled water just before using. 

 These are washed off in distilled water or merely dried with blotting paper and 

 double stained in a saturate aqueous solution of methylen blue, which too is 



