1974 



Journal of Applied Microscopy 



Note on a Method of Cultivating Anaerobic Bacteria. 



There is a very excellent method of growing anaerobes which I have not 

 seen mentioned, or have overlooked in Hunziker's recent articles in the Journal 

 OF Applied Microscopy ; a combination of the pyrogallol method and a vacuum. 

 Plates or tubes are placed in a bell jar with stop-cock at the top (Fig. 1) and 

 this is connected with a vacuum pump. Pyrogallic acid is placed on the floor 



r\ 



1 



L. 



Pig. 1. — Bell jar sealed to dish with paraffin. 



Fig. ■>. 



of the dish and the illustrated glass apparatus (Fig. 2), which can be easily 

 made by any one familiar with blow-pipe work, inserted, after being filled with 

 KOH or NaOH. The vacuum causes the NaOH in the tube to syphon off 

 and the last remaining trace of oxygen is absorbed. F. C. Harrison. 



Ontario Agri. College. 



An Easy Method of Demonstrating Gas Production in 



Test-Tubes. 



A fair sized piece of absorbent cotton is placed in each test-tube of sugar 

 (lactose, glucose, etc.) bouillon. The cotton should not be forced to the bottom 

 of the tube or packed tight. The tubes when sterilized are ready for use and 

 are inoculated in the ordinary manner. Gas formed by the growth of the 

 bacteria in the bouillon is caught among the fibres of the absorbent cotton and 

 the gas bubbles, whether large or small, are very clearly demonstrated. 



Ontario Agri. College. F. C. HARRISON. 



