2034 Journal of Applied Microscopy 



Relation to air — Aerobic and facultative anaerobic. 

 Relation to gelatin — Slow liquefier. 

 Color — Varies from white to orange. 

 Stain — Stains very easily with ordinary aniline dyes. 

 Gelatin : Stick culture — Slight plumose growth along needle track. Lique- 

 faction infundibuliform, at first dry with a yellowish deposit. 

 Gradually liquefaction spreads, becoming horizontal, and a cloudy 

 liquid is formed with heavy orange precipitate. 

 Plate culture — Surface col.; at first a whitish bead, smooth at center, 

 becoming yellowish with age. Liquefaction slight or none. Colony 

 about 5 mm. in diameter. 

 Agar: Streak culture — A white to yellowish, glistening, smooth, moist, 



abundant growth along line of needle. 

 Milk: Curdles in three days at 37° into a soft curd with amphoteric reac- 

 tion. No subsequent digestion, but a clear, watery fluid settles out on 

 top. Occasionally a slight digestion. 

 Potato : A dry, granular, raised growth, not spreading. Color varies from 



pure white to orange. 

 Smith lube : No gas produced in either glucose or lactose. 

 'Synthetic Media : Reduction of nitrates marked. 

 Bouillon : In two days becomes cloudy. After four weeks, a yellow sediment 



is deposited, but the liquid is still cloudy. 

 Conn, in speaking of this micrococcus, considers it one of the most important 

 of dairy species, and suggests that it likely exists in the milk ducts. 



VI. B. No. 18, Conn (?). 



Occurrence : Almost constantly present in all samples. 



General Characters : Shape and arrangement — Bacillus occurring singly and 

 in twos. 



Size — Short and thick, about one and one-half times long as broad. 



Motility — None. 



Spore formation — Terminal spores found in old gelatin and agar cultures. 



Relation to temperature — Grows at room temp., but optimum is 37°. 



Relation to air — Aerobic and facultative anaerobic. 



Relation to gelatin — Non-liquefier. 



Color— White. 



Stain — -Stains evenly without ordinary aniline dyes. 

 Gelatin : Stick culture — An abundant plumose growth along needle track, 

 with a thick, white, cream-colored surface growth. 



Plate culture — Surface col.; an opaque white bead about 1 mm. in 

 diameter, smooth and round. 

 Agar : Streak culture — An abundant thick, white, moist growth. 

 Milk : No effect is produced on milk in three weeks. After four weeks it 



becomes slightly acid, and a slow coagulation takes place. 

 Potato : Slight light brown, or cream-colored growth, raised, but not spreading. 

 Smith lube : No gas produced in either glucose or lactose. 



