2036 Journal of Applied Microscopy 



General Characters : Shape and arrangement — Bacterium occurring singly. 

 Size — Very short, but thick. 

 Motility — Actively motile. 

 Spore formation — None. 



Relation to temperature — Grows at room temp., but optimum is 37°. 

 Relation to air — Aerobic and slightly facultative anaerobic. 

 Relation to gelatin — Slow liquefier. 

 Color— White. 



Stain — Stains evenly with aniline dyes. 

 Gelatin : Stick culture — Slight beaded growth along needle track. Lique- 

 faction at first crateriform, and then horizontal, a white scum being 

 formed on top, and a white deposit on the bottom. 

 Plate culture — Surface col.; large, round, flat, white, regular colony, 

 with a dense, white, nuclear center, and a clearer outer zone. 

 Under the microscope the nucleus is granular and the outer zone 

 reticulated, except the narrow boundary margin, which is structure- 

 less. 

 Agar: Streak culture — An opaque, porcelain colored, moist, glistening, 



slightly spreading growth. 

 Milk: Milk rendered alkaline and slightly curdled in three days at 37°, and 



then slowly digested. 

 Potato : At first a moist spreading growth, scarcely discernible. Afterwards 

 it becomes thicker, brownish, but remains moist. It is not reticulated. 

 Smith Tube : No gas produced in either lactose or glucose. 

 Synthetic Media : Slight reduction of nitrates. 



Bouillon : In one day at 37° becomes turbid. In four weeks there is a white, 

 irregular scum on top and a white deposit. After six weeks the liquid 

 becomes clear. 

 The gelatine plate colony appears very similar to No. VII, but the organisms 

 otherwise differ, both morphologically and culturally, in many particulars. Like 

 No. VII, it appeared quite constantly for some weeks, but since then has disap- 

 peared. 



IX. B. exigiium. Wright (19). 



Occurrence : Found quite constantly in the milk of one cow. 



General Characters : Shape and arrangement — Bacterium occurring singly. 



Size — Medium size, about two and one-half times as long as broad. 



Motility — None. 



Spore formation — None. 



Relation to temperature — Slow growth at room temp., optimum growth 

 at 37°. 



Relation to air — Aerobic and facultative anaerobic. 



Relation to gelatin — Slow liquefier. 



Color — Ferruginous to light red. 



Stain — Stains evenly with ordinary aniline dyes. 

 Gelatifi : Stick culture — Slight arborescent growth along line of needle, but 



