-^038 Journal of Applied Microscopy 



whole center of about 5 mm. becomes filled with granular masses 

 which frequently project beyond the rim. 

 Agar: Streak culture — An abundant, raised, porcelain white, moist growth, 



not spreading. 

 Milk: Slight effect. A slight white deposit is formed in litmus milk, and 

 after a long period of 4 or 5 weeks the milk becomes thinnish. Reaction 

 alkaline. 

 Potato : A beautiful opaque, porcelain white, dry chain of bead-like, much 



raised colonies. 

 Smith lube : No gas produced in either glucose or lactose. 

 Synthetic Media : No reduction of nitrates. 

 Bouillon: In one day, at 37°, becomes cloudy. In six weeks it is still 



cloudy, and there is a slight white deposit. 

 This coccus comes in the same class as Conn's 167, and may be identical 

 with it. The most marked variations are the gelatin colony, and the fact that 

 in no culture of this germ was there the slightest indication of a yellow color. 

 At the same time it agrees in morphological characters, and especially in the 

 fact that, although a liquefying coccus, it fails to curdle milk. 



Like several of the forms previously described, this coccus was found to be 

 present for some time in the samples taken, but afterwards in a few weeks com- 

 pletely disappeared. In no cases was it present in large numbers. 

 Ontario Agr. College. F. C. HARRISON, 



M. ClTMMING. 



An Improved Method for Making Collodion Tubes for 



Dialyzing. 



The use of a collodion tube for dialyzing is so convenient, so cheap, and so 

 accurate as to commend itself for more general use, both to the chemist and to 

 the biologist. 



The following method of preparing these I have found much the easiest, and 

 by it tubes of any desired size may be made very rapidly. Into test tubes, or 

 tubes of that pattern of the desired size, pour enough three per cent, collodion 

 so that by inclining the tube and twirling it the sides will be completely coated. 

 Pour back the superfluous collodion, still twirling the tube a few seconds to 

 allow the collodion film to begin drying. Turn the tube mouth down over a 

 coarse screen, or prop it up on two blocks, so that the excess collodion may 

 drain easily and also to allow free access for the air to dry and harden the film. 

 Allow to stand from five minutes to one hour, then fill the tubes with water, and 

 in a few minutes the collodion tube is loosened and may be easily drawn out 

 from the test tube and is ready for use. The longer drying makes a tougher 

 tube, the shorter drying a quicker one. A much thicker solution of collodion 

 may be used if stronger and slower tubes are desired. These tubes will keep 

 for some time in water. If exposed long to the air, they become shrunken and 

 rather brittle. Karl Kellerman. 



Bureau of Plant Industry. 



