2044 Journal of Applied Microscopy 



7. Methyl Violet and Rubidin. 



Griesbach (1890) stained dry blood films shortly with methyl violet, rubidin ; 

 washed, dried, mounted, 



8. Safrinin. 



Leigh (1888) allowed air-dried preparations to swim face downward in a half 

 saturated solution of safrinin in absolute alcohol. 



9. Tbionin. 



Marchoux (1897) gives the following formula for a thionin stain for malarial 

 blood : 



Concentrated alcoholic solution of thionin (60 per cent, alcohol), 20 c. c. 

 2 per cent, watery solution of carbolic acid, - - - 100 c. c. 

 This mixture is used after standing fifteen days, then it stains in a few 

 seconds. The leucocytes and parasites are stained red-violet. 

 Rees (1901) gives this formula : 



Thionin blue, 1.5 grams 



Absolute alcohol, - - - - - 10 c. c. 



Aqueous solution of carbolic acid, 5 per cent. 100 c. c. 



Keep for two weeks, or longer. Filter before use, and dilute with distilled 

 water 1 in 4. Staining takes place in about 10 minutes. Protoplasm of mala- 

 rial parasites stains as intense purple, nuclei of leucocytes blue, red corpuscles 

 a faint greenish blue. 



Craig (1901) gives still another formula after Futcher : 



Saturated solution of thionin in 5 per cent, alcohol, 20 



Two per cent, carbolic acid, . . . . . 100 

 The solution should stand several days before using. Red corpuscles stain 

 a slight greenish hue, parasites a deep violet. 



10. Toiuidin. 



Rees (1901) gives the following formula for toiuidin blue stain for malarial 

 blood : 



Toiuidin blue, 5 grains 



Sodium carbonate, ----- 4 " 



Distilled water, - - - - - - 100 c. c. 



Dissolve the sodium carbonate in water gently warmed, stir in the toiuidin 

 blue. Keep a few days, and filter before using. Stain 30 seconds. 



Massachusetts State Board of Health. ErneST L. Walker. 



