and Laboratory Methods. 



2049 



ELEMENTARY MEDICAL MICRO-TECHNIQUE. 



For Physicians and Others Interested in the Microscope. 



Copyrighted. 



X. EXAMINATION OF THE BLOOD. 



It is only within the last few years that so-called " Blood work " has been 

 done by the medical profession in general. The diagnostic value of blood exam- 

 inations is well established 

 and instruments for the 

 purpose are now found in 

 the office of every pro- 

 gressive medical man. 



A general examination 

 of the blood should in- 

 clude an estimation of the 

 blood cells both red and 

 white, estimation of the 

 haemaglobin, a general 

 microscopical examination and an examination for the plasmodium of malaria. 



For the estimation of the blood cells, commonly called a blood count, a 

 Thoma-Zeiss Hsemacytometer with pipettes for both red and white corpuscles 

 and the following solutions will be required : 

 1 oz. 95 per cent, alcohol. 

 1 oz. Sulphuric ether. 

 1 oz. Toison's fluid, which is made as follows : 



Thoma-Zeiss Haemacytometer, for counting red and white 

 blood corpuscles. 



Sodium sulphate 

 Sodium chloride 

 Glycerine, pure 

 Aqua distilled 

 Methyl violet 



Normal Human Blood. Stained with haema- 

 toxylin and eosin. Red cells and poly- 

 nuclear leucocytes are shown magnified 

 1200 diameters. y'.-inch oil immersion 

 objective, Bausch & Lomb compensating 

 photo ocular No. 2. 



S grams 



1 gram 

 30 grams 

 - 160 c. c. 

 - .025 grams 

 Clean the finger or lobe of the ear 

 with soap and water, alcohol, and lastly 

 with ether. Dry thoroughly and punc- 

 ture with a blood lancet. The drop of 

 blood must exude without pressing the 

 tissues. Wipe away the first blood, 

 using the second drop for the count. 

 Suck blood into the pipette marked 1 

 and 101, up to the mark 1. Wipe off 

 the adherent blood on the point and 

 draw the pipette full of Toison's solu- 

 tion to the mark 101, thus filling the 

 bulb of the instrument completely. 

 Shake well that the glass ball may thor- 

 oughly mix the blood and the salt solu- 

 tion. One-half of this mixture should 

 be blown out of the instrument. A 



