and Laboratory Methods. 2061 



CURRENT ZOOLOGICAL LITERATURE. 



CHARLES A. KOFOID, University of California. 



Books and Separates of Papers on Zoological Subjects should be Sent for Review to Charles A. 

 Kofoid, University of California, Berkeley, California. 



Prowazek, S. Zur Entwicklung der Gegarinen. The gegarines Monocystis agilis and 

 Arch. f. Protistenkunde, 1 : 299-305, Taf. 9, j^ ^^ ^g^e obtained from the testis 



1902. ° 



of the earthworm. The testis was dis- 

 sected out and fixed in corrosive-acetic. Sections were stained in haematoxylin- 

 rubin, in Ehrlich's triacid stain, and in iron haematoxylin and orange G. In 

 Ehrlich's triacid the protoplasm of the sporozoite stains red and the nucleus bluish 

 green. Fine nuclear details are best secured with the iron haematoxylin. Cover- 

 glass preparations by the Ehrlich method were also stained in the triacid stain 

 with differential results. Romanowski's method gave no satisfactory prepara- 

 tions of this material. c. a. k. 



Goodrich, E. S. On the Structure of the Ex. The " kidney " of Amphioxus may be 

 cretory Organs of Amphioxus. Quart. Journ. demonstrated when living material is 

 Mic. Sci. 45: 493-503, pi. 27, 1902. ° 



at hand. The specimen is pinned out 



on its back in a shallow dish of sea water. The atrium is ripped up with a 

 needle along the mid-ventral line and the two metapleural folds pinned aside- 

 The exposed pharynx is then also ripped up with a needle and portions of the 

 right or left side of the pharynx can then be torn out with the forceps and placed 

 on a slide, care being taken to lay the outer side uppermost. Permanent whole 

 mounts or sections may be made of these parts by the usual methods. Details 

 of structure in the fresh material must be studied with oil-immersion and high eye- 

 pieces. The author concludes that the so-called kidney of Amphioxus does not 

 open into the coelom by ccelomic funnels, as described by Boveri. The so-called 

 thread cells resemble the solenocytes of annelids, in fact the whole organ is much 

 like the nephridium of some annelids, for example of the Glyceridae. c. A. k. 



„. „ , , , r , • J T^ • Amceba miurai Ijima has been found 



Jniura, K. Amoebenbefund in der Panctions- 

 fliissigkeit bei Tumoren der Peritonealhohle. in three cases of peritoneal tumors in 

 Mitth. Med. Fac. Kais. Jap. Univers. 5: j^an in the fluid exudate drawn from 

 15 pp., 1900. 



the body cavity. Upon standing the 



fluid separates into a yellowish upper layer and dark red lower one in which are 

 white and red blood corpuscles, fibrin clots, tumor cells, and the structures 

 regarded as amoebae. The fluid was drawn with aseptic precautions and kept 

 at the temperature of the body during examination under the microscope by 

 means of a warming apparatus. Nuclei were demonstrated by acetic acid and 

 preparations made by drying the fluid upon the slide and staining in hEematoxylin. 

 The consistency of the organisms is greater than that in most amoebseand move- 

 ments are very slow. They affect principally the villi on the knoblike protuber- 

 ance and sometimes take the form of wave-like changes in the surface. Many 

 moribund and disintegrating individuals occur with the living ones. This 

 Amceba is reported in the exudate associated with tumors of three very different 

 types. c. A. K. 



