2062 Journal of Applied Microscopy 



Rbssler, P. Ueber den feinern Bau der Cys Cysticerci were killed by suspending 

 ticerken. Zool Jahrb, Abth. f. Anat u. Ontog, j-^em scolex downward in corrosive 

 10 : 423-449, Tar. 29, 30, 1902. 



sublimate. Pressure of the bladder 



fluid secures extension of the tissues. The addition of acetic acid to the subli- 

 mate is not advantageous. Alcohol as a killing fluid destroys the cuticula. 

 Flemming's fluid causes great blackening of the bladder, but not of the scolex, 

 where there is little fat. Sections 3-5 pi in thickness can be cut without decal- 

 cification. Weigert's fibrin stain, which yields excellent results with trematodes, 

 stains only the basement membrane. Eosin-iron hsematoxylin was used for the 

 excretory system, Van Gieson's method for the parenchyma, and toluidin blue for 

 epithelial cells and their prolongations. This last stain is used in warm con- 

 centrated solution in warm oven until an intense blue color is secured. The 

 slide is washed in distilled water and then in 10 per cent, ammonium 

 molydate, which prevents loss of color in subsequent dehydration. Methylen 

 blue was used intra 7>itam in weak solution (1:2000) injected into the bladder. 

 The worm should be removed from the cyst to warm normal salt solution and 

 kept for about an hour at 17-18° R. when the first traces of coloration appear. 

 Pieces for examination are cut from the bladder wall and placed, cuticula upper- 

 most, upon a glass cell and covered with cover-glass. The air has free access 

 to the under surface, which is not under pressure. Such preparations may be 

 examined for hours with immersion objectives without loss of color, which occurs 

 when sections are covered in the ordinary way. c. a. k. 



These bear animalcules were found in 

 Scbaudinn, F. Die Tardigraden. Fauna Arc- ,, . 1 • r 1 



tica, 2: iSs-rge, 1901. the collections made m fresh water, m 



slime, and in moss from arctic shores. 

 The moss-dwelling forms may be thrown into a state of turgor and quiescence 

 prior to killing by drying slowly and then plunging into water. They recover 

 their locomotor powers in several days. The fresh water Macrobiotus withstands 

 such treatment only in the egg. Tardigrades, along with Protozoa, nematodes, 

 and rotifers may be removed from the moss by comminution, immersion in 

 water and decantation of the lighter detritus. The animals will be found in the 

 heavier material and may be quickly gathered by means of a centrifuge. Tardi- 

 grades are well fixed by sublimate-alcohol (saturated aqueous solution of sublimate 

 2 parts, absolute alcohol 1 part), or in 10 per cent, formalin, though the latter 

 decreases their stainability. The author did not find that the tardigrades were 

 difficult to stain, as have most other investigators of the group. In 40 per cent, 

 alcoholic alum-carmine and in diluted Grenacher's (Delafield's) hsematoxylin the 

 sublimate material stained readily without the necessity of puncturing the 

 body wall. Unstained individuals were mounted in potassium acetate in which 

 claws and mouth parts are more clearly shown than in glycerine. Mounting in 

 balsam was accomplished without the shrinkage usually found. This was 

 obviated by slow and careful dehydration in the centrifuge. The author com- 

 mends this instrument very highly for carrying small objects en masse through 

 the successive steps of preparation for microscopical examination. c. a. k. 



