2090 



Journal of Applied Microscopy 



ELEMENTARY MEDICAL MICRO-TECHNIQUE. 



For Physicians and Others Interested in the Microscope. 



Copyrighted. 



XL 



HAEMAGLOBIN. 



To estimate the Haemaglobin, clean the finger or lobe of the ear with soap 

 and water, alcohol and ether, dry and puncture with the blood lancet. Fill 



the graduated pipette of a Gower's 

 Haemaglobinometer to the 20 cmm. 

 mark. Put in the graduated tube a 

 little distilled water and discharge 

 the blood from the pipette in it, wash 

 out the pipette with a little distilled 

 water, discharging this into the mix- 

 ture in the tube. Add water drop by 

 drop, shaking the tube each time until 

 the color matches the color in the 

 gelatine tube. The colors match in 

 ' daylight. Holding a piece of white 

 paper behind the tubes assists in 

 matching the colors. The percentage 



Gower's Haemaglobinometer. 



of haemaglobin is read direct from the height of the fluid in the graduated 

 tube. Normal blood seldom reads higher than 95 to 98 per cent. 



Fleischl's Haemometer is a more ac- 

 curate instrument for estimating the Haema- 

 globin. Draw the blood as described. 

 Apply one of the small metal handled 

 pipettes to the blood drop so that it is filled. 

 Empty, and wash out the blood in one side 

 of the comparison cell which has previously 

 been half filled with distilled water. When 

 removing the tube wash it with a few drops 

 of distilled water, which should go in with 

 the other water. Mix the blood and water 

 thoroughly with the metal handle of the 

 pipette. Now fill both compartments level full with distilled water, adjust the 

 wedge underneath, and set the comparison cell on the stage of the instrument so 

 that the side filled with water only may be above the colored wedge of the 

 instrument. Examine at once in a darkened room with artificial light (candle or 

 gas) as the colors do not match by daylight. By means of the milled head the 

 wedge may be adjusted until the colors of the two compartments match. The 

 percentage of haemaglobin is read from the graduated scale — 95 to 98 per 

 cent, haemaglobin representing normal blood. It is seldom that blood is 

 found that reaches 100 per cent. 



Fleischl's Haemometer. 



