2100 Journal of Applied Microscopy 



to pure paraffin was reduced to a few seconds to prevent shrinkages. The author 

 urges the value of many series for comparison to eliminate individual peculiari- 

 ties, of the full study, careful delineation and measurement of embryos prior to 

 sectioning, and of the graphic and plastic methods of reconstruction in embryo- 

 logical investigation. Some of the excellent drawings were outlined on blue- 

 prints, which were washed out and then finished as desired. c. a. k. 



llliogworth, J. F. The Anatomy of Lcucapina The killing fluid used was Van Gehuch- 

 cremilata. Zool. Jahrb., Abth. f. Anat. u. ten's alcohol-acetic-chloroform mixture 

 Ontog. 16: 449-491, pls. 31-33, 1902. j^ ^^^^^^ ^^^ ^^^^^^^^ ^j^^j^^l ^^^ ^^ 



placed by 95 per cent. This was injected through the buccal sinus. This fluid 

 whitens the muscular tissues, while the nerves retain their yellowish tint. For the 

 finer nerves 5 to 10 per cent, nitric acid was similarly injected and the animals were 

 then macerated for a month or more in weak nitric acid in the light. This gives 

 a yellowish tint to the muscles, leaving the nerves a lighter hue. For nerve dis- 

 secting a glass tube was used drawn to a capillary point and bevelled. This is 

 attached to a, hydrant by a rubber tube. The sharpened end is used as a dis- 

 secting needle and a stream of water passing through it serves to remove the 

 debris of dissection. c. a. k. 



M «< Ti. J TT 1. J- T- • 1 , , Embryos of the trout (Triitta fario) 

 Moroff, Theodor. Ueber die Entwicklung der -^ \ J 1 



Kiemen bei Knochenfischen. Arch. f. mik. killed at intervals of one to four days 



Anat. und Entwick.,60: 428-459, Taf. 21, were used for this investigation. Picro- 

 22, 1902. ° 



acetic, chrom-acetic, and sublimate 



were used for fixing, the last giving the best results. Thin sections were double 



stained in haematoxylin-eosin or picro-acid fuchsin. Sublimate material stained 



with the former was well differentiated, the cartilage staining a dark violet, the 



other tissues a violet-blue, and the blood corpuscles red. The chrom-acetic 



material was somewhat better preserved, but stained with difficulty. c. a. k. 



Q. , M 1 ^. T- , TA , r Effffs of Z. anatifera in all of the early 



Bigelow, M. A. The Early Development of °° -^ ^ 



Lcpas, a Study of Cell-lineage and Germ- Stages of development were secured 

 layers. Ball. Mus. Comp. Zool.. Harv. 40: j^om adults on drifting timber at 

 61-144, pis. 12, 1902. ° 



Wood's Hole in August. Early stages 



of L. fasciolaris were rarely found. Development from egg to nauplius takes 

 place in the mantle chamber, the eggs being found in the cavities of the egg 

 plates between the body and the mantle. The eggs are conveniently fixed in 

 situ in the plates. Only reagents containing picric acid were satisfactory. 

 Kleinenberg's stronger fluid and a saturated solution of picric acid in 35 per 

 cent, alcohol gave excellent fixation. A saturated solution of picric acid in 5 per 

 cent, acetic acid gives superior results in penetration and subsequent transpa- 

 rency of the eggs. Less than 5 per cent, acetic acid fails in penetration. 

 Mercuric chloride caused opacity, but was valuable in differentiating yolk in 

 subsequent staining. Entire eggs from the picro-acetic material were stained 

 in Grenacher's alcoholic (35 per cent.) borax carmine, decolorized in 70 per cent, 

 alcohol -|- 0.3 per cent. HCl. Clove oil and especially cassia oil gave the best re- 

 sults as clearing agents. Sections were stained in diluted Delafield's haematoxylin 

 and counterstained for yolk with eosin or orange G. c. a. k. 



