2106 Journal of Applied Microscopy 



and not to secondary contamination of the vaccine sores. He finds that the 

 larger part of them came from the virus from one maker, and concludes that it is 

 probably due to the rare case of the contamination of the calves from which 

 the virus was taken with the tetanus bacillus. A final foot-note states that Dr. 

 R. W. Wilson has succeeded in actually finding the tetanus bacillus in some of 

 the same vaccine used at the time of the chief outbreak in 1901. h. w. c. 



Von Wendt. Ueber eine einfache Methode, The heating bacteria in the ordinary 



Bakterien ohne Trocknen an Deck — oder fixing method undoubtedly produces 



Obiectglaser zu fixieren. Cent. f. Bac, ,. , , ,._ ^- • ^i 



I 31* 671 1Q02. slight modifications in the organism so 



that the subsequent microscopic appear- 

 ance is somewhat modified. The author has devised a method of fixing the bac- 

 teria without the use of heat in order to facilitate the careful microscopic study. 

 The method is briefly as follows : A loopful of bacteria culture is placed in a 

 drop of water or in water containing 3 per cent, nitric acid and one-half per cent, 

 corrosive sublimate. A cover-glass is then covered with a thin layer of Meyer's 

 albumin glycerine fixative, a drop of water is placed upon the cover glass 

 and a small amount of the bacteria mixture is placed carefully upon the drop of 

 water with the platinum loop. The bacteria spread through the drop and grad- 

 ually sink. The whole is placed under a watch glass for half an hour when the 

 bacteria will have sunk to the bottom of the drop. It is then placed, still uhder 

 the watch glass, in an oven at the temperature of 75° for 8 to 10 minutes to coag- 

 ulate the albumen. This must be done under conditions which prevent the 

 water from evaporating in order to keep the bacteria moist. The water may 

 then be removed and staining reagent applied, for the bacteria will be fixed by 

 the albumen. h. w. c. 



Drigalski and Conradi. Ueber ein beifahren ^he authors have devised a method of 

 zum Nachweis der Typhusbacillen. Zeit. f. distinguishing typhoid bacillus based 

 yg. u. nee. .23,1902. upon somewhat different principle from 



any of the other methods used. It is dependent upon the use of a culture medium 

 which especially stimulates the growth of the typhoid bacillus, the essential 

 features of which are the use of a larger per cent, of agar and bouillon than 

 usual, the presence of an alkaline albuminate (nutrose) and of crystals violet B. 

 The medium is made with 3 pounds of chopped beef, 2 liters of water, 20 grams 

 of peptone, 10 grams of salt, 20 grams of nutrose, and 60 of agar. A second 

 solution contains 260 c. c. of litmus solution and 30 grams of milk sugar. These 

 two solutions are mixed and rendered alkaline, after which 20 c. c. of a one- 

 tenth solution of crystal violet B is added. The typhoid colonies develop read- 

 ily on such plates and are easily differentiated by the agglutination test after 24 

 hours' growth. With this material he is able to separate the typhoid bacillus in 

 a large number of cases from the body excretions before there are distinct symp- 

 toms in the patient and in many cases where the Widal test has failed. 



H. w. c. 



Castellani. Upon a Special Method for the This method depends upon a dilution 

 Detection of the Typhoid Bacillus in the of the blood sufficiently tO remove the 

 Blood. Cent.f. Bac. u. Par. 1,31: 0,477- disturbing factor of the agglutinating 

 bodies. A few centimeters of blood of the patient is added to several large 

 flasks each containing 30 c. c. of faintly alkaline beef broth. The flasks are 

 incubated at blood heat, and in 12 cases out of 14 the typhoid bacillus was detected 

 by subsequent study. h. w. c. 



