68 Bacterial TUkflit of Fear Blossoms in Sout/t Africa 



sixth day the agar, which was a neutral litmus tint when planted with 

 the organism, is Killarney green, when viewed by transmitted light 

 facing the streak ; viewed by transmitted light facing the side of the 

 slant the colour is deep red, nearest Nopal red. By reflected light the 

 upper part of the agar is still green, but from the deeper layers there 

 is a distinctly purplish light reflected. There was no further change 

 in colour during the six weeks the tubes were kept under observation. 



Loffier's blood serum is not liquefied, a streak culture on this medium 

 is similar to that on nutrient agar. 



Cabbage agar. On this medium the organism forms a ribbon-like 

 streak, honey yellow, 3 — 4 mm. broad, and with a wrinkled shagreen 

 surface. 



Nutrient gelatine (+ 15). Colonies at 20° C. attain a diameter of 

 about 4 mm. in three days; they are thin, spreading, with a slightly 

 irregular margin, in the centre of each colony there is a small, glisten- 

 ing, raised point ; the remainder of the growth has a dull, ground glass 

 appearance. Submerged colonies are small, yellowish opaque. 



There is no liquefaction, and colonies do not change further, except 

 to increase in size in thinly sown plates. There is no fluorescence in 

 this medium. 



Nutrient gelatine stab. There is a growth similar to the gelatine 

 colonies, covering the surface of the medium and a filamentous growth 

 along the upper part of the medium ; but no liquefaction during two 

 months and no development in the depths of the medium. 



Nutrient gelatine streak is flat, about 4 mm. broad with undulate 

 or crenate margin ; here also the growth is dull and thin, and has the 

 appearance of ground glass when held up to the light. 



Nutrient bouillon (+ 15) is heavily clouded in 24 hours at 20° C, 

 and afterwards becomes very turbid. There is a tendency to pellicle 

 formation, but when disturbed the pellicle breaks up into flocculi and 

 sinks. A greenish fluorescence may be observed, and is usually very 

 noticeable especially from the surface of the medium. A considerable 

 amount of rather viscid sediment collects at the bottom of the tube or 

 flask. 



Nutrient bouillon + 2 % dextrose. The growth in this medium is 

 heavier than in nutrient broth to which no sugar had been added, but 

 the broth does not become fluorescent. 



Nitrate bouillon becomes heavily clouded, and there is a thick 

 pellicle, but the fluorescence is not marked. 



Dunhanis solution is liQt a very favourable medium and does not 



