168 Ustuliiia Zoiiata {Lev.) Sacc. on Hevea Bmsiliensis 



wood on which the fungus was growing in pure culture in slight cuts 

 made with a sterile knife, either in the collar or woody parts of the stem. 

 Control plants were kept; these were treated in the same way, but small 

 pieces of newly sterilised wood were placed in the cuts. The results are 

 placed in tabular form below. 



Date 

 examined 



No. 

 24. vii. 15 

 27. vii. 15 

 29. vii. 15 

 11. viii. 15 



5, X. 15 



6. X. 15 



U {r) at 

 collar 



U (r) in 

 in stem 



U (s) at 

 coUar 



U (.9) in 

 in stem 



1* 2* 3* 4* 

 + 



6* 



8* 



+ 



+ 



+ + 



+ 



+ 



* Nos. 1, 2, 3 and 7 were dead on the date of examination. The death symptoms were 

 typical, the leaves suddenly wilting. When the plants were cut open at the point of inocu- 

 lation the progress of the fungus for one or two inches above and below this place was 

 clear, and black lines were forming in the diseased tissues from the wounded places. 

 No. 5 gave a similar result, but the plant was examined before the leaves wilted. Nos. 4 

 and 8 showed the fungus progressing in the tissues on the date of examination, while No. 6 

 completely recovered. Cuts made in control plants all healed quickly. 



Expt. 2. In this experiment, similar inoculations were carried out on 

 five year old, untapped trees growing in an area clear of all rotting 

 timber. Eighteen trees were inoculated, nine with U (r), nine with U (s). 

 Six each of U (r) and U (s) were made at the collar, the bark being lifted 

 with a sterile knife, and a small piece of culture wood inserted. Six 

 inoculations were made in the branches, three with U (r), three with 

 U (s). These were made by cutting deeply into the cortex and inserting 

 similar pieces of culture wood as those used in the collar inoculations. 

 Inoculations made: 22. vi. 15, and examined: 1. ii. 16. 



Tree U (r) at Tree U (r) in Tree U (s) in Tree U (s) at 

 No. collar No. branches No. branches No 



+ 



+ 



The tabulated results of Experiment 2 show that twelve out of 

 eighteen inoculations were successful. The branch inoculations were 

 uniformly good, both with U (r) and U (s). Seven out of twelve root 

 inoculations were successful. Considering the difficulties attached to 

 root inoculations, this result is most conclusive. 



