E. S. Salmon and H. Wormald 9 



appear. Later the latter were further developed and on September 29 

 were used for a second series of inoculations on fig-trees. Two of the 

 "tendrils" were removed on a sterile hooked platinum wire and placed 

 in a small tube of sterile distilled water which was then agitated in order 

 to difiuse the conidia throughout the liquid. Wounds are made on 

 branches as in Exp. I, but instead of inserting agar and mycelium, a 

 drop of the water containing the conidia was transferred to the wound 

 by means of a pipette (previously sterilized) and allowed to flow in 

 between the bark and the wood. In the case of control wounds a 

 drop of sterile water only was inserted in a similar manner. Some of 

 the wounds were bound round with damp cotton wool (previously 

 sterilized in autoclave) and tinfoil secured by raffia in order to ensure 

 favourable conditions for the germination of the conidia : the other 

 wounds were left uncovered. In other cases again water containing 

 conidia and fragments of tendrils was dropped on damp cotton wool 

 which was then bound round certain internodes with tinfoil and raffia 

 as before, but without injuring the bark. 



Eight of the largest branches on two young fig-trees, var. White 

 Marseilles, were selected and treated as follows, the branches being 

 from 0-8 cm. to 2 cm. in thickness. 



No. 1. Inoculated; wound covered with cotton wool and tinfoil. 



„ 2. As in 1. 



,, 3. Inoculated ; wound left uncovered. 



,, 4. Control; not inoculated but branch cut and wound covered 

 with cotton wool and tinfoil. 



„ 5. Control as in 4. 



,, 6. Control; wound left uncovered. 



„ 7. Spores placed on damp cotton wool which was then wrapped 

 round an internode. 



„ 8. As in 7. 

 Eight branches were similarly selected on two young trees of 

 the Brown Turkey variety and respectively treated as those of 

 the White Marseilles. Thus in this experiment six branches were 

 wounded and inoculated, with an equal number of control branches 

 also wounded, and in four cases conidia were applied to uninjured 

 branches. 



To test the viability of the conidia used in the experiment a drop 

 of the conidia-containing water (as used in the inoculations) was used 

 in preparing a "poured plate" which was then placed in the greenhouse 

 in close proximity to the trees. No growth was to be observed in the 



