H. WORMALD U)l 



A])ril 27. The styles of the inoculated flowers were withered and brown 

 to the base and the stamens had collapsed. In the corre- 

 sponding flowers (of the same age) on the control trusses 

 the styles were not discoloured and the stamens were upright. 

 ,, 29. All the flowers of the inoculated trusses were drooping and 

 the leaves were beginning to wilt; the stamens of the un- 

 treated flowers were however rigid (not collapsed with 

 distorted filaments as in the two inoculated flowers). 



May f . All the leaves of trusses {a) and (c) were by this time curled, 

 brown and withered; the stamens of the non-inoculated 

 flowers were still rigid. (This stage is shown in Figs. 3 

 and 5.) 

 ,, 5. From truss (c) the disease had traversed the tissues of the 

 spur and was invading the main stem as shown by a slight 

 sinking of the bark at the base of the spur and extending 

 about half the way round each side; truss (6) was just 

 beginning to wilt, indicating that the transpiration current 

 w^as being interrupted, and that the vessels of the xylem 

 were attacked. 

 ,, 10. The canker at truss (c) had almost girdled the stem and 

 extended 0-5 cm. upwards and 1-5 cm. downwards from the 

 base of the spur; the cankered area was indicated by a 

 distinct wrinkling of the bark whict was also slightly de- 

 pressed below the general level. 

 ,, 11. The stem was now completely girdled; the lowest branch 

 distal to the affected spurs began to wilt. 

 19. The leaves of the other two branches above the spur were 

 drooping and turning brown. 



June 2. A canker had also developed from truss (a) so that by this 

 date the two cankers had united and together they extended 

 along the stem for a distance of 10 cm., i.e. from 4 cm. 

 above (a) to 6 cm. below (c) ; truss (d) however was still 

 alive. Towards the upper end of the canker there was a 

 series of dark lines more or less parallel to one another giving 

 a zonate appearance. That portion of the tree above the 

 infected spurs was by this time cjuite dead. (This stage is 

 shown in Figs. 4 and 6.) 



Dec. 6. Young pustules were bursting through the bark at the lower 

 end of the canker ; the immature conidia remained cohering 

 in chains when mounted in water. 



13—2 



