30 



SfnrUes hi Bacteriosis 



clear bright amber colour, wliile in cell B it retained a lairly strong 

 fuchsin stain. We have, then, what appears to be a series of stages in 

 the disappearance of such granules as are represented in Fig. 3. The 

 nature of these granules is not definitely established, but a micro- 

 organism has been isolated from diseased tissue with which these bodies 

 compare closely in size, and since bacteria in the diseased tissue are 

 indicated in no other way and, moreover, the granules stain deeply 



Fit 



4. Drawn witli the aid of the camera lucida I'ruin a hand section through a "blister- 

 sjjot" on the leaf of Protea cynaroidefi. The cells shaded darkly contain a more or less 

 granular matter embedded in a gum-like matrix, and they retain a fuchsin stain in 

 proportion to the degree of granulation. The cells shaded uniformly contain structure- 

 less transparent masses of gum which reacted vaiiously with fuchsin, some, as cell A, 

 lost all the stain and possessed a clear amber colour after treatment of the section 

 with 50 per cent, alcohol, while neighbouring cells, as cell B, retained a fairly strong 

 fuchsin stain. 



with carbol fuchsin and Victoria blue, the assumption of their bacterial 

 nature seems amply justified. It is not always possible to demonstrate 

 in sections of diseased tissue such bacteria-like granules, but in all cases 

 masses of the granular gummy substance occur in various degrees of 

 granulation and of staining capacity, and the degree of granulation 

 always runs pari passu, with the power of retention of a bacterial stain. 

 One is therefore led to assume that bacteria entering the cell are early 



