36 



Studies in Bacteriosis 



number, 10-20/x in length and are unipolar (Fig. 6). The organism is 

 therefore a Pseudomonas. Motility soon ceases in cultures on solid media, 

 even after 24 hours at 22° C. only a few individuals are found in motion ; 

 in liquefied gelatine and in bouillon, however, motility was observed in 

 cultures of three days incubation at the above temperature. 



Staining. Positive results were obtained with the usual bacterial 

 stains and with Gram's stain, negative results with spore stains and 

 capsule stains. 



10/x 



Fig. 6. Drawn with the aid of the camera lucida from a preparation of Pseudomoim^ 

 Proteamaculans stained by the method of Plimmer (unpublished). 



(ii) Cultural Characters. 



The organism grows luxuriantly on solid media of various composition 

 and of varying H ion concentration up to about + 30 of Fuller's Scale. 



Gelatine streak + 10. Incubated at 22° C. Liquefaction 4 mm. wide 

 and 2 mm. deep after 24 hours; complete liquefaction after 72 hours. 



Gelatine stab + 10. Incubated at 22° C. Liquefaction at first crateri- 

 form, the (-rater being 5 mm. wide and 2 mm. deep after 24 hours; later, 

 i.e. after 3 days, infundibuliform with a layer of liquid gelatine 6 mm. 

 deep above, the funnel being 7 mm. across at the top and 3 mm. at the 

 bottom. 



