S. G. Paine and W. F. Bewley 193 



B. Cultural Characters. 



In all cases, unless otherwise stated, the incubation temperature has 

 been 22° C. 



Plate culfrires in bouillon agar + 10. The colonies on poured plates 

 show after 24 hours; surface colonies have a diameter of | to 1 mm., 

 depth colonies are lenticular and just visible with a lens. The surface 

 colonies are round, raised, wet-shining, with a tendency to excentric 

 development from a depth colony. The colour is a mid-chrome by 

 reflected and somewhat milky by transmitted light. 



10^ 



Fig. 5. Showing Bacillus lathyri stained by Steven's modification 

 of van Ermengem's method. 



Streak cultures on bouillon agar + 10. The streak is raised, wet- 

 shining, butyrous yellow, with entire margin. 



Stab cultures on bouillon agar + 10. Growth is visible along the stab 

 after two days as a narrow yellow streak right to the bottom of the stab. 

 The margin is slightly echinate. The surface colony after five days has 

 a diameter of 4 mm. and a bright yelloAV butyrous appearance. 



Plate cultures on bouillon-gelatine + 10. Colonies appear on the 

 second day, are 2 or 3 mm. in diameter on the third day, and show no 

 sign of liquefaction for 8-10 days. A liquid halo then appears and 



