S. G. Paine and W. F. Bewley 195 



only brown. On titration after two months' incubation the original 10 cc. 



N 

 required 1-3 cc. of — . NaOH for neutralisation against phenolphthalein 



as against 1-0 cc. required by the control. This small degree of acidity 

 and small development of gas were consistently observed but they do 

 not appear to justify the classification of the organism as a glucose 

 fermenter. They possibly arose from some impurity in the sample of 

 peptone employed although a control tube of peptone without sugar 

 did not give this result. The organism was still motile in the brown 

 broth when examined after 26 days. 



10 'per cent, peptone + 2 per cent, lactose. The colour remained 

 neutral. A strong ring formed but no pellicle. 



10 per cent, peptone + 2 per cent, sucrose. Precisely the same as in 

 case of glucose above. 



Bouillon + 2 per cent, mannite. Became acid on the fifth day. No 

 gas formed. 



Bouillon + 1 per cent, nitrate. Slightly turbid on the first day and 

 nitrite was present. On the second day the nitrite was strongly positive 

 and remained so for two months. Turbidity was never very strong, 

 no ring or pellicle was produced but the broth became somewhat 

 mucoid. 



Potato plug. About 1 cc. volume of the plug taken from half-way 

 down ground in 200 cc. of water on addition of 1 cc. of iodine gave a 

 bright red colour, no trace of starch remained. Diastatic action is there- 

 fore strong. 



Uschinshf solution. Distinctly turbid on the second day, a ring had 

 formed and the colour was neutral. On the fifth day the litmus had 

 begun to bleach, a strong yellow ring had formed and a loose pellicle 

 which was easily broken up. After twenty days the litmus had become 

 completely bleached, a strong yellow ring and pelhcle were formed and 

 a large yellow deposit filled the lower half of the tube. This was very 

 mucus-like and spread on a slide almost like a sputum. As stated above 

 capsules were suspected to be present, especially in the yellow rino-, 

 although no demonstration of a capsule could be obtained. 



Dunhani's solution. Became turbid on the second day, a loose 

 yellow pellicle formed later. Indol was tested for on the thirteenth day 

 but was not present. 



