S. G. Paine and W. F. Bewley 197 



been attempted as yet for the reason that the identity of the organism 

 with B. h(l/ii/ri was only discovered after this paper had been sent to 

 the press. Experiments to settle this point will be made next summer. 



INOCULATION EXPERBIENTS. 



Preliminary Experiments. 



28. 5. 19. Six young tomato plants (Comet variety) were used. The stems were 

 washed with alcohol and then with sterile water. A small piece of culture 

 was jjricked in near the top of the plant and the wound covered with 

 tm-foil to avoid secondary infection. 



31. 5. 19. Brown corky patches appeared round the stab. 

 11. 6. 19. Black furrows up and down the stem from the stab. Three 

 inches up and two down. 



28. 6. 19. Typical black fuiTows on the stem up and down from the 

 wound. Leaves browned in patches. General appearance of striped 

 plants. 



Three controls were perfectly healthy. 

 28. 5. 19. Six young plants as in the previous experiment were used. Inoculations 

 were made by pricking in a piece of culture near the base of the plant and 

 covering as before. 



14. 6. 19. Black fm'rows eight inches up the stem. Lower paii' of leaves 

 attacked. 



30. 6. 19. Plants smothered in lesions. 

 Three controls were perfectly healthy. 

 28. 5. 19. Six plants removed from the soil and their roots well washed. They were 

 then washed in alcohol followed by sterile water. They were inoculated by 

 I'ubbing a piece of culture on the roots, and were then re-planted. 



15. 6. 19. First brown patch appeared on the stem about half an inch 

 above the soil level, 



29. 6. 19. Lesions on the stem seven inches from the ground. 

 5. 7. 19. Plants badly attacked. 



Three controls quite healthy. 

 5 7. 19. Organism re-isolated from representative members of each experiment. 

 3. 6. 19. Four plants two feet high were inoculated by rubbing a piece of culture 

 on a rough leaf-base such as is left by careless pruning. 



8. 6. 19. Bases blackened and furrows appear which run from the base 

 to the stem. 



15. 6. 19. Furrows down the stein two inches below the inoculated base. 

 3. 7. 19. Fiu-rows up and down the stem. First and second pair of 

 leaves above and the first pair below the point of inoculation attacked. 

 Two controls healthy. 

 3. 6. 19- Four plants two feet high were used. Several badly striped leaves were 

 crushed in a mortar and the juice rubbed into a carelessly pruned leaf- 

 base. 



11. 6. 19. Bases blackened. 



