198 



Studies in Bacteriosis 



30. 6. 19. Typical stripe furrows running from the bases into the stem 

 were visible. 



21. 7. 19. The plants were all badly striped. 

 Four controls made by rubbing the juice of healthy leaves into jagged 

 leaf -bases were perfectly healthy. 

 3. 6. ] 9. Four green tomato fruits were inoculated by pricking in a small piece of 

 culture after sterihsing the outside. 



10. 6. 19. Brown ring round the prick. 



17. 6. 19. Brown sunken patch round the prick. The fruit was cut 

 open when it was found that the tissue round the stab was browned. A 

 brown patch was present in the loculus wall, from which the organism 

 was re -isolated. 



The control fruit showed the wound healed and no sign of stripe. 

 20. 7. 19. The organism re-isolated from inoculated plant on 5. 7. 19 was pricked 

 into six twelve inch plants. 



29. 7. 19. Typical furrows on stem near the wound. 



4. 8. 19. The organism was again isolated and found to be identical 

 with the original. 



The effect of mmiuring upon the results of artificial infection. 



A standard soil compost was made up as follows: 

 72 cubic feet of virgin soil, 

 32 lbs. lime, 

 8 ,, bone meal, 

 8 ,, bone flour. 

 72 twelve inch pots were each given one cubic foot of the compost. 

 To these pots nitrogen in the form of ground hoofs and potash as sul- 

 phate of potash were added in varying amounts. Each series consisted 

 of eight pots planted with uniform tomato plants of the variety Comet. 



Series 1. 1 c. ft. f()inj)ost and I Ih. hoofs ])(•!• \w\, 



KaS04 



No potash 



No nitrogen 

 hoofs and I Ih. KoHO., per pot 



Complete 



28. 5. 19. Six plants of each series were inoculated by "'pricking in" some of the 

 culture into the lower portion of the stem and covering the wound with a 

 piece of tin-foil to prevent secondary infection. Two plants of each scries 

 were left as controls. They were pricked with a sterile needle and covered 

 in the usual wav. 



